南极适冷菌Pseudoalteromonas sp.S-15-13胞外多糖EPS-Ⅱ对小鼠S_(180)肉瘤抑制作用的研究  

Inhibitory effects of exopolysaccharide(EPS) from an Antarctic psychrotrophs Pseudoalteromonas sp.S-15-13 on the sarcoma_(180) of mice

作　　者：李江[1] 陈靠山[1] 李光友[1] 刘少芳[2] 刘占涛[3] 

机构地区：[1]国家海洋局第一海洋研究所海洋生物活性物质重点实验室,山东青岛266061 [2]山东大学生命科学学院,山东济南250100 [3]青岛大学医学院,山东青岛26603

出　　处：《中国海洋药物》2007年第3期9-13,共5页Chinese Journal of Marine Drugs

基　　金：国家863计划项目(2002AA244031)

摘　　要：目的研究南极适冷菌Pseudoalteromonas sp.S-15-13胞外多糖EPS-Ⅱ的抑瘤作用。方法不同浓度的EPS-Ⅱ腹腔注射于荷S180肉瘤小鼠,通过荷瘤小鼠的脾重和瘤重变化来测定EPS-Ⅱ的抑瘤效果;采用免疫组化方法,测定荷瘤小鼠增殖细胞核抗原(PCNA),三联组氨酸(FHIT)和S-100蛋白阳性表达的情况。结果(1)不同剂量的EPS-Ⅱ均具有提高荷瘤小鼠脾脏重量,有效抑制肿瘤生长的作用,最高抑瘤率可达45.1%。(2)免疫组织化学结果表明,不同剂量的EPS-Ⅱ均能抑制PCNA表达,促进FHIT和S-100蛋白表达的作用。结论南极适冷菌S-15-13胞外多糖EPS-Ⅱ可能通过调节PCNA,FHIT和S-100的表达来发挥抑瘤作用。Objective To investigate the effects of exopolysaccharides （EPS） purified from an Antarctic psychrotrophs Pseudoalteromonas sp. S-15-13 on the S180 of mice. Methods After different doses of EPS- Ⅱ were injected into abdominal cavity of mice implanted with sarcoma 180 cells for 10 days. The tumor and spleen of mice were weighed and the positive expressions of PCNA, FHIT and S-100 were measured by immunochemistry. Results （1） EPS-Ⅱ could significantly increase the weight of spleen and inhibit the growth of S180 of mice with highest percentage inhibition of 45. 1%. （2） Immunohistochemistry analysis showed that EPS-Ⅱ could significantly inhibit the expression of PCNA and enhance the expressions of FHIT and S-100 protein. Conclusion EPS- 1I might inhibit the growth of S180 through modulating the expressions of PCNA, FHIT and S-100.

关 键 词：南极适冷菌 胞外多糖 免疫功能 

分 类 号：R931.711[医药卫生—生药学] R965[医药卫生—药学]