褐藻酸降解菌的筛选及产酶条件优化  

Screening of alginate producing strain and optimization of fermentation condition

作　　者：庞敏[1] 王常红[1] 韩宝芹[1] 刘万顺[1] 

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《中国海洋药物》2007年第3期34-40,共7页Chinese Journal of Marine Drugs

摘　　要：目的通过对褐藻酸钠的降解实验,从病烂海带上筛选出降解能力较高的菌株,从而制取褐藻酸酶。方法经过筛选,选出产酶活力高的s4菌株,优化产酶条件,制取粗酶,并对酶的性质进行初步研究。结果s4菌株最佳产酶条件是AlgNa 1.2%,NH4Cl 0.9%,NaCl 1.5%,pH=7.5,温度在25℃最佳。Abstract.Objective A strain with high alginase activity was screened and isolated by decomposing sodium alginate from the decaying parts of brown alga Laminaria japonica and Undaria pinnatifida, in order to produce alginase. Methods The strain s4 with high alginase activity was chosen by filtration. The alginase producing media was optimized and the alginase was produced and its characterization was investigated. Results The optimum fermentation conditions for alginate lyase producing as follows, media AlgNa 1.2 %, NH4 Cl 0.9 %, NaCl 1.5 %, pH= 7.5,and temperature 25℃. Conclusion The alginase produced by strain s4 showed high alginase activity and good stability.

关 键 词：褐藻酸酶 培养条件优化 酶学性质 

分 类 号：R93[医药卫生—生药学] TQ460.6[医药卫生—药学]