高耐毒性酿酒酵母的紫外诱变选育  

Mutagenesis and selection of high toxic tolerance Saccharomyces cerevisiae by the ultra-violet radiation

作　　者：王璞[1] 田沈[1] 王丹[1] 张兰波[1] 杨秀山[1] 

机构地区：[1]首都师范大学生命科学学院,北京100037

出　　处：《可再生能源》2007年第3期31-33,38,共4页Renewable Energy Resources

基　　金：国家"863"计划资助项目(2002AA514010和2001AA514024);北京市教委项目

摘　　要：木质纤维素生产乙醇已成为世界各国研究开发的热点。但在酿酒酵母对木质纤维素稀酸水解产物的乙醇发酵中,对水解产物中的毒性物质非常敏感。菌种对水解液毒性物质耐受力相对较低是影响木质纤维素乙醇发酵工业化的主要因素之一。利用紫外线对酿酒酵母进行诱变,得到2株高效耐水解液中毒性物质突变株k和n。2株突变株发酵未脱毒的木屑稀酸水解产物的乙醇产量分别达到理论值的71.0%和61.3%,为进一步提高酿酒酵母耐毒性的研究和木质纤维素稀酸水解生产乙醇的工业化提供了基础。Ethanol is the new type of fuel which is researched by countries all over the world due to its high thermal efficiency, clean and nonpolluting characters as well as safety characters. Nowadays, toxic-tolerance ability of the yeasts was lower, which was one of the key factors restricting the development of fermentative ethanol producing industry. UV was used to irradiate Saccha- romyces cerevisiae in this experiment, and 2 mutants were gains, namely k and n.These two mutants would provide material for discuss the mechanism of the toxic tolerance of Saccharomyces cerevisiae and industry production of the bioethanol.

关 键 词：酿酒酵母 紫外诱变 耐毒性 乙醇发酵 

分 类 号：TK6[动力工程及工程热物理—生物能] Q813[生物学—生物工程]