高效液相色谱与电喷雾电离质谱联用测定婴儿配方奶粉中的牛磺酸和维生素  

Taurine and vitamine detection in infant milk powder by HPLC-ESI/MS

作　　者：陈稚[1] 揭新明[1] 蔡康荣[1] 

机构地区：[1]广东医学院分析中心,广东湛江524023

出　　处：《广东医学院学报》2007年第3期252-254,共3页Journal of Guangdong Medical College

摘　　要：目的利用高效液相色谱与电喷雾电离质谱联用技术,建立一种同时测定婴儿配方奶粉中的牛磺酸和4种维生素(维生素B1、维生素B5、维生素B6、维生素PP)含量的分析方法。方法色谱条件采用RP-C18色谱柱,流动相为5mmol/L七氟丁酸和甲醇,梯度洗脱。质谱采用电喷雾正离子模式(ESI+),以马尿酸为内标定量。结果4种维生素和牛磺酸含量测定方法的线性关系良好(r=0.9996～0.9999,最低定量限10μg/L),前处理技术简单,用该法测定了婴儿配方奶粉中的牛磺酸和4种维生素的含量,回收率为99.1%～101.7%。结论本方法操作简便,精密度好,结果准确可靠。Objective To establish a method for detecting taurine, and vitamin B1, B5, B6 and PP in infant milk powder by high-performance liquid chromatography and electrospray ionization/mass spectrometry （HPLC-ESI/MS）. Methods The gradient elution separation of RP-CI8 column was made by 5 mmol/L heptafluorobutyric acid and methanol, respectively. Target components were determined by ESI-MS in positive mode （ESI ＋ ）, and hippuric acid was used as an internal standard. Results There was a good linear correlation in the detection of taurine and 4 vitamins in infant milk powder. Conclusion HPLC-ESI/MS is a simple, accurate and reliable method for taurine and vitamine detection in infant milk powder.

关 键 词：高效液相色谱 电离质谱 婴儿配方奶粉 牛磺酸 维生素 

分 类 号：R155.57[医药卫生—营养与食品卫生学]