壳聚糖球固定化酵母细胞的研究  

Immobilization of Saccharomyces Cerevisiae Cells Using Chitosan Beads Carrier System

作　　者：李桂银[1,2] 黄可龙[1] 蒋玉仁[1] 丁萍[1] 刘艳飞[1] 

机构地区：[1]中南大学化学化工学院 [2]湖南科技职业学院高分子材料与工程系 长沙 410004

出　　处：《现代生物医学进展》2007年第7期981-984,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金(20376085)

摘　　要：以戊二醛为交联剂,将壳聚糖球交联引入醛基,然后将交联的壳聚糖球浸泡在酵母细胞悬浮液中,制备了固定化酵母细胞壳聚糖球。以苯乙酮酸为底物,催化合成了D-扁桃酸。最优固定化条件是戊二醛的质量分数w(GA)=1%,酵母细胞与交联壳聚糖球的质量比m(Y):m(CB)0=0.5,交联时间为6h,固定化时间为18h,底物浓度为10mmol/L,在此条件下反应最大转化率和产物光学纯度分别高达67.86%和98.05%ee。固定化酵母壳聚糖球具有良好的重复使用性和贮存稳定性。Saccharomyces cerevisiae cells were immobilized onto the crosslinked chitosan beads containing aldehyde groups that were prepared in the presence of glutaraldehyde. Asymmetric microbial reduction of phenylglyoxylic acid to D-mandelic acid with immobilized cells was studied. The optimum conditions of immobilization were 10/0 w （GA） of glutaraldehyde mass fraction, 0.5 of yeast cells/beads weight ratio, 6h of the crosslinking time, 18h of the immobilization time and 10 mmol/L of substrate concentration. The conversion ratio and optical purity of the product were as high as 67.86% and 98.05%ee, respectively. The immobilized yeast cells on chitosan beads have good reusability and storage stability.

关 键 词：酵母 球形壳聚糖 固定化细胞 D-扁桃酸 

分 类 号：Q939.5[生物学—微生物学]