大麦穗废弃物中提取β-葡聚糖的初步研究  

Preliminary Study on the Extracting of β-Glucan from Barley Spike's Waste

作　　者：刘献文[1] 沈寿国[2] 宋建民[2] 胡博[2] 郑敏[2] 张洁[2] 蔡敬民[1] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036 [2]合肥学院生物与环境工程系,安徽合肥230022

出　　处：《大麦与谷类科学》2007年第2期38-40,共3页Barley and Cereal Sciences

基　　金：安徽省优秀青年科技基金项目(04043051);安徽省科技厅年度重点攻关项目(0523074);合肥学院科学研究发展基金项目(05ky002zr)

摘　　要：以大麦穗废弃物为原料,通过碱液浸提、胰酶水解和乙醇、硫酸铵、异丙醇的先后沉淀,提取纯化β-葡聚糖。分别以自制β-葡聚糖和进口β-葡聚糖为底物,用β-葡聚糖酶活测定比较法,得出自制β-葡聚糖纯度为77.12%。淀粉碘液显色反应结果表明淀粉含量少,还原糖仅为0.83%,自制糖中蛋白质含量平均占5.91%,β-葡聚糖提取率平均为1.08%,最高达1.44%。β -Glucan was extracted and pured from harley spike＇s waste, and its process contained extracting in alkali liquor, hydrolyzed by Trypsinase and deposited in ethanol, ammonium sulphate, isopropanol in turn. The purity of β - glucan was 77.12% used β - glucan activity measurement method which took self - made β - glucan and imported β - Glucan as suhstrate. The results of starch - iodine solution color reaction showed that it contained less starch, 0. 83% of reducing sugar, average 5.91% protein in self- made sugar, and the average extraction rate was 1.08% , 1.44% was the highest.

关 键 词：大麦穗废弃物 Β-葡聚糖 胰酶 

分 类 号：S512.3[农业科学—作物学]