槟榔提取物体外诱导人口腔黏膜成纤维细胞增殖模型的建立  

Proliferation Model of Human Oral Mucosal Fibroblasis Induced by Extract from Areca Nut in Vitro

作　　者：谭劲[1] 李元聪[1] 陈明[2] 陈安[2] 彭楚湘[2] 

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学

出　　处：《湖南中医药大学学报》2007年第3期11-13,共3页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金项目(30472231)

摘　　要：目的为研究口腔黏膜下纤维化的修复机制,建立体外人口腔黏膜成纤维细胞增殖模型。方法体外培养口腔黏膜成纤维细胞,并用波形蛋白、角蛋白免疫细胞化学方法鉴定;分别用0、5、50、100、150、200μg/mL浓度的槟榔提取液对培养的成纤维细胞诱导,MTT法检测细胞增殖。结果波形蛋白表达阳性,而角蛋白表达阴性;50、100μg/mL槟榔提取物促进口腔黏膜成纤维细胞增殖。结论培养的细胞为纯化的成纤维细胞,槟榔提取物诱导口腔黏膜成纤维细胞增殖的最佳浓度为50～100μg/mL。该模型可为进一步的研究提供实验基础。Objective To provide a proliferation model of human oral mucosal fibroblasts for the study of repair mechanism on oral submucous fibrosis. Methods Human oral mucosal fibroblasts were cultured in vitro and identified by vimentin and kieratin immunochemistry method; the culturing fibroblasts were induced by areca nut extract （ANE） with different concentration （0,5,50,100,150 and 200 μg/mL）; and then the fibroblasts proliferation was detected with MTT assay. Results The expression of the vimentin was positive while the keratin negative, and ANE with concentration 50 μg/mL and 100 μg/mL significantly promoted the fibroblasts proliferation. Conclusion The cultured cells are purifying human oral mucosal fibroblasts, the appropriate concentration for ANE inducing proliferation of oral mucosal fibroblasts are 50 μg/mL-100 μg/mL. This model can be a basis for the farther study of oral submucous fibrosis repair mechanism.

关 键 词：槟榔提取物 口腔黏膜 成纤维细胞 增殖 

分 类 号：R781.5[医药卫生—口腔医学]