甘肃野生酵母菌株的鉴定及抗性评价  

Identification and resistance evaluation of wild yeast strains from Gansu Province

作　　者：谢荣[1] 王庆国[2] 刘建民[2] 陆璐[1] 赵长增[1] 刘天明[2] 

机构地区：[1]甘肃农业大学农学院,甘肃兰州7300702 [2]山东轻工业学院食品与生物工程学院,山东济南250353

出　　处：《甘肃农业大学学报》2007年第3期114-118,共5页Journal of Gansu Agricultural University

基　　金：山东省自然科学基金(Y2005D15).

摘　　要：采用形态和生理生化鉴定方法及26S rDNA D1/D2区序列分析,对甘肃部分县市的17份土样中分离到的29株野生酵母菌株进行了鉴定.结果显示,29株酵母归为10属18个种,其中优势属为隐球酵母属(4个种),假丝酵母属(4个种),毕赤酵母属(3个种);中国新记录种7个,包括Williopsis californica,Williopsis californicavar.dimennae,Cryptococcus aerius,Candida pseudolambica,Candida fermentati,Hanseniaspora occidentalis,Pichia pijperi.同时对这29株酵母进行了抗性评价,菌株38A(Candida tropicalis)耐60%的葡萄糖,45℃高温和14%NaCl.29 wild yeast strains isolated from 17 soil samples collected from some counties in Gansu Province were identified through morphological, physiological,and 26S rDNA D1/D2 domain sequence a- nalysis. The results indicated 29 wild strains belong to 10 genera, 18 species. Among them, the dominant genera were Cryptococcus （4 species）,Candida （4 species）,Pichia （3 species）;7 species were Chinese new records,including Williopsis cali f ornica , Williopsis cali f ornica var. dirnennae, Cry ptococcus aerius , Candida pseudolarnbic ,Candida f errnentati , Hanseniaspora occidentalis , Pichia pijperi. At the same time, the resistance of 29 wild strains were evaluated as well. Strain 38A（Candida tropicalis identified） could grow in media with high osmotic pressure （glucose 60 %,NaCl 14 %）,and at high temperature（45 ℃）.

关 键 词：野生酵母 鉴定 26S RDNA D1/D2区 抗性 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]