小肝细胞癌患者染色体1p36杂合性缺失的特点  

Loss of heterozygosity at chromosomal 1p36 in small hepatocellular carcinoma patients

作　　者：董辉[1] 丛文铭[1] 冼志红[1] 吴伟清[1] 王艳华[1] 吴孟超[1] 

机构地区：[1]第二军医大学东方肝胆外科医院病理科,上海200438

出　　处：《中国肿瘤生物治疗杂志》2007年第3期225-229,共5页Chinese Journal of Cancer Biotherapy

基　　金：国家自然科学基金资助项目(No30370645)~~

摘　　要：目的:探讨人类染色体1p36等位基因杂合性缺失在小肝细胞癌(small hepatocellular carcinoma,sHCC)发生发展中的作用及其与临床病理表现的关系。方法:采用PCR-非变性聚丙烯酰胺凝胶电泳和硝酸银染色技术,对140例信息性sHCC中1p36上9个多态性微卫星标志位点的杂合性缺失(loss of heterozygosity,LOH)进行检测。结果:本组140例sHCCs发生LOH的总频率为65%(91/140),LOH以D1S507最高,为40.8%(31/76);在肿瘤直径≤1cm的HCC中,D1S507和D1S2893位点的LOH发生率显著高于直径>1cm组(P<0.05);在Edmondson分级≥Ⅲ级的HCC中,D1S468、D1S2694和D1S243位点LOH发生率明显高于≤Ⅱ级的HCC(P<0.05);包膜完整HCC中D1S243和RIZ位点的LOH发生率明显低于有包膜突破的HCC(P<0.05);女性患者中RIZ位点LOH发生率明显高于男性患者(P<0.05)。结论:sHCC在人染色体1p36上存在多个LOH位点,并与临床病理学参数之间有一定的相关性,提示这些缺失区可能存在候选肿瘤抑制基因,并与sHCC的发展和演进过程有关。Objective： To investigate loss of heterozygosity （LOH） on the chromosomal region 1p36 in small hepatocellular carcinoma （sHCC） and discuss the association between microsatellite alterations and clinicopathological parameters of sHCC. Methods ：The PCR-SSCP based microsatellite polymorphism analysis technique was performed on 140 informative cases of sHCC by using a panel of 9 polymorphic microsatellite markers in 1p36. Results： Ninety-one of informative 140 cases （65%） showed LOH on at least one locus. LOH was found most frequently at D1S507 （40.8%, 31/76）. LOH on D1S507, D1S2893 was more frequent in tumors less than 1 cm in size （P 〈 0.05 ）. Similarly, LOH on D1S468, D1S2694 and D1S243 was frequently detected in cases with Edmondson grade higher than Ⅲ （ P 〈 0.05 ）. LOH on D1S243, RIZ occurred more frequently in cases with absent or partially encapsulated tumor than in those with intact tumor capsule （P 〈 0.05 ）. LOH frequency on RIZ was significantly higher in female patients than in male （P 〈 0.05 ）. Condusion： High frequent LOH on chromosome 1p is a common molecular event in sHCC. LOH at some particular loci is associated with certain clinicopathological parameters of sHCC, suggesting that some putative tumor suppressor genes may exist in these regions, which may play a role in the development and progression of sHCC.

关 键 词：肝细胞癌 杂合子缺失 DNA 微卫星 等位基因 

分 类 号：R730.231[医药卫生—肿瘤]