嗜酸乳杆菌的筛选及培养基的优化  

Screen and study on culture medium of multiplying of Lactobacillus acidophilus

作　　者：张智[1] 余萍[1] 

机构地区：[1]东北林业大学食品与科学,哈尔滨150040

出　　处：《中国乳品工业》2007年第6期25-27,共3页China Dairy Industry

摘　　要：通过模拟正常人体胃内状态,筛选出10株耐酸耐胆盐的嗜酸乳杆菌。针对嗜酸乳杆菌的营养需要,采用正交实验优化基础培养基及增殖因子的用量,得出最佳基础培养基配比:蛋白胨1%,葡萄糖0.5%,大豆低聚糖1.5%,酵母膏1.0%(均为质量分数);最佳增殖因子添加量为西红柿汁12%。最后使用优化出的增菌培养基测定生长曲线,同时测定pH值和OD值的变化确定增菌培养终止时间为16h,通过平板菌落计数,此时菌数可达2.2×1012mL-1。In this article ,we choose ten acid and bile salt tolerance Lactobacillus acidophilus through intimating state of people＇s stomach. According to the nutritional needs of Lactobacillus acidophilus basis culture medium and growth promoting factors were optimized by quadratic design. The optimum basis culture medium composition was as following：1.0% peptone, 0.5% glucose, 1.5% soya oligofructose, 1.0% yeast extract. The optimum growth promotingfactors were as following： 12% tomato juice. Growth curve with optimized stimulatedculture were determined by following the change ofpH and OD. According to the growth curve, the terminal time was at 16 hours. Lactobacillus acidophilus got to 2.2×10^12 cfu/mL in this culture for 16hours at 37 ℃.

关 键 词：嗜酸乳杆菌 耐酸耐胆盐 培养基优化 

分 类 号：Q93-331[生物学—微生物学] Q93-335