牛磺酸对大鼠幽门结扎型胃溃疡的影响  

Effect of taurine on gastric ulcer in pylorus-Iigated rats

作　　者：余良主[1] 王帮华[1] 黄碧兰[1] 唐琼[1] 丁洁琼[1] 

机构地区：[1]湖北省咸宁学院医学院生理教研室,湖北省咸宁市437100

出　　处：《世界华人消化杂志》2007年第13期1545-1548,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨牛磺酸对大鼠幽门结扎型胃溃疡的影响及其机制.方法:采用结扎大鼠幽门的方法建立大鼠胃溃疡模型.45只Wistar大鼠随机分为三组,即正常对照组、溃疡模型组和牛磺酸处理组.经6 h后各组大鼠处死,检测三组大鼠胃黏膜溃疡指数(UI)、胃液总酸度、胃蛋白酶活性和壁细胞H^+,K^+-ATP酶活性.结果:与正常对照组相比,溃疡模型组大鼠UI值(35.3±3.7 vs,P<0.01)和胃液总酸度增大(28.56±3.81 mmol/L vs 20.34±4.40 mmol/L,P<0.01),同时胃液胃蛋白酶活性[7.58±1.58μg/(mL·min)vs 5.83±1.22μg/(mL·min),P<0.01]和壁细胞H^+,K^+-ATP酶活性升高(8.86±1.50 U/mg vs 6.95±1.03 U/mg,P<0.01);而应用牛磺酸则减轻了大鼠应激性胃黏膜损伤,UI值(15.4±3.6 vs 35.3±3.7,P<0.01)和胃液总酸度减小(19.58±3.68 mmol/L vs 28.56±3.81 mmol/L,P<0.01),胃液胃蛋白酶活性[6.36±1.45μg/(mL·min)vs 7.58±1.58μg/(mL·min),P<0.05]和壁细胞H^+,K^+-ATP酶活性(7.62±1.46 U/mg vs 8.86±1.50 U/mg,P<0.05)降低.结论:牛磺酸具有抗大鼠幽门结扎型胃溃疡的作用,其机制可能与抑制胃酸及胃蛋白酶分泌有关.AIM： To explore the effect of taurine on the pylorus ligation-induced gastric ulcer in rats and its mechanism. METHODS： Rat model of gastric ulcer was made by pylorus ligation method. A total of 45 Wistar rats were randomly and averagely divided into 3 groups： normal control group, ulcer model group and taurine-treated group. Six hours later, all the rats were killed, and the gastric mucosal ulcer index （UI）, total acidity of gastric juice, pepsin activity and H^±,K^± -ATPase activity in parietal cells were measured. RESULTS： In comparison with the normal control group, the ulcer model group showed an increase in UI （35.3 ± 3.7 vs O, P 〈 0.01）, total acidity of gastric juice （28.56 ± 3.81 mmol/L vs 20.34 ± 4.40 mmol/L, P 〈 0.01）, pepsin activity （7.58 ±1.58 pg/（mL· min） vs 5.83 ± 1.22 μg/（mL · min）, P 〈 0.01） and H^＋, K^＋ -ATPase activity of parietal cells （8.86 ± 1.50 U/rag vs 6.95 ± 1.03 U/mg, P 〈 0.01）. However, in the treated group, the gastric mucosal injury was attenuated, and the value of gastric mucosal UI （15.4 ± 3.6 vs 35.3 ± 3.7, P 〈 0.01）, total acidity of gastric juice （19.58 ± 3.68 mmol/L vs 28.56 ± 3.81 mInol/L, P 〈 0.01）, pepsin activity [6.36 ± 1.45 μg/（mL · min） vs 7.58 ± 1.58 μg/ （mL · min）, P 〈 0.05] and H^＋, K^＋ -ATPase activity of parietal cells （7.62 ± 1.46 U/mg vs 8.86 ± 1.50 U/mg, P 〈 0.05） were decreased significantly as compared with those in the model group. CONCLUSION： Taurine can alleviate gastric ulcerative injury in pylorus-ligated rats, which may be related to the inhibitory effect of taurine on the secretion of gastric acid and pepsin.

关 键 词：大鼠 胃溃疡 幽门 牛磺酸 胃酸 胃蛋白酶 ATP酶 

分 类 号：R573.1[医药卫生—消化系统]