应用二次回归正交旋转组合设计提取桑叶多糖的研究  

Study on the extraction of polysaccharides from mulberry leaves by orthogonally rotational combination design

作　　者：邢东旭[1] 廖森泰[1] 刘吉平[2] 邹宇晓[1] 吴娱明[1] 徐玉娟[1] 刘学铭[1] 

机构地区：[1]广东省农业科学院蚕业与农产品加工研究所广东省农产品加工公共实验室,广东广州510610 [2]华南农业大学动物科学学院,广东广州510642

出　　处：《食品工业科技》2007年第6期145-147,共3页Science and Technology of Food Industry

基　　金：广东省自然科学基金重点项目(05100575);广东省科技攻关项目(2004B20401004)。

摘　　要：采用二次回归正交旋转组合设计方法研究了温度、时间、料水比、pH对桑叶水溶性多糖提取率的影响,确定了桑叶多糖的较优提取范围。实验结果通过贡献率法计算表明,在实验范围内各因子对桑叶多糖提取率作用的大小依次为:料液比>温度>pH>时间;频率分析法得到桑叶多糖最优提取工艺为:温度85℃,时间90min,料水比1∶35,pH为7.45,在此工艺条件下桑叶多糖提取率为4.69%。Orthogonally rotational combination design was used to investigated the effect of the four-factor （temperature, time, solvent and solid ratio, pH） on the water-soluble polysaccharides of mulberry leaves. The results indicated that extraction ratio of TPM was affected obviously in successive order by solvent and solid ratio, temperature, pH and time. The best extraction conditions were as follows： temperature 85℃, time 90min, solvent and solid ratio 1：35 and pH7.5. The yield of the polysaccharides of mulberry was 4.69% under above conditions.

关 键 词：桑叶多糖 二次回归正交旋转组合设计 提取率 优化 

分 类 号：TS201.1[轻工技术与工程—食品科学]