金属元素对Xhhh菌株表达MnP酶及降解污染物的影响  

Effects of Metals on MnP Expression and Wastewater Degradation Efficiency of Xhhh Strain

作　　者：赵大勇[1] 于洪峰[2] 潘文扬[1] 孙石磊[1] 张徐祥[1] 朱程军[1] 肖琳[1] 崔益斌[1] 程树培[1] 

机构地区：[1]南京大学污染控制与资源化研究国家重点实验室,南京210093 [2]中国矿业大学环境与测绘学院,江苏徐州221008

出　　处：《土壤》2007年第3期448-452,共5页Soils

基　　金：国家教育部博士点基金项目(20030284038);国家863项目(2001AA214191)资助。

摘　　要：测定分析了制药废水中Mn2+、Cu2+、Zn2+和Se4+4种金属元素离子水平对跨界原生质体融合(IKPF)特效菌株Xhhh表达锰过氧化物酶(MnP)水平及降解有机污染物效率的影响。结果表明,影响MnP表达水平的金属元素离子顺序为Mn2+>Se4+>Zn2+>Cu2+。在MnP水平与生物比降解率(SDR)之间,存在极显著的正相关性(P<0.01)。研究结果为应用IKPF-Xhhh特效菌株处理制药废水的工艺设计提供了重要的分子生物学参数。本文还对Xhhh在土壤修复中的应用前景进行了分析讨论。Effects of 4 metal ions, Mn^2＋, Cu^2＋, Zn^2＋ and Se^4＋, were determined on level of manganese peroxidase （MnP） expression and degradation efficiency of Strain Xhhh, an inter-kingdom protoplast fusaht （IKPF） was constructed for degrading organic pollutants in wastewater from a pharmaceutical plant. Results showed that in terms of specific activity （MnP-SA） level, the four metals were in the order of Mn^2＋〉Se^4＋〉Zn^2＋ 〉Cu^2＋. A significantly positive correlation （P〈0.01） was observed between the MnP-SA level and the specific degradation rate （SDR）. The findings provided some important molecular biological parameters that were useful in designing wastewater treatment technology using the IKPF-Xhhh strain. The prospect of application of Strain Xhhh to soil hioremediation was also discussed.

关 键 词：制药废水 金属元素 生物降解 MNP SDR 土壤修复 

分 类 号：X172[环境科学与工程—环境科学]