通过基因枪轰击转化获得转基因小麦植株的研究  

TRANSGENIC WHEAT PLANTS BY MICROPROJECTILE BOMBARDMENT OF IMMATURE WHEAT EMBRYOS

作　　者：黄粤[1] 柯遐义[1] 李宝健[1] 

机构地区：[1]中山大学生物工程研究中心,广州510275

出　　处：《西北植物学报》1997年第2期142-146,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金："863"高科技项目资助

摘　　要：利用JQ－700型高速基因枪将pDM302质粒DNA上的bar基因即PAT酶（phosphinothricinacetyltransferase）基因导入了冬小麦品种“农大146”的幼胚中。经过在含有phosphinothricin（简称ppt）的选择培养基上筛选，得到了9块具有ppt抗性的愈伤组织。PCR电泳检测与PCR－Southern杂交结果显示，外源bar基因已转化进了由其中4块愈伤组织再生出的转基因的小麦植株中。The bar gene (phosphinothricin acetyltransferase gene) in plasmid pDM302 hadbeen transferred into the immature embryos of a winter wheat cultivar (Triticum aestivum L.) 'Nong Da 146' by microprojectile bombardment with JQ-700 system.Afterselection for 2-3 months,we have obtained 9 pieces ppt (phosphinothricin)-resistant calluses.PCR electrophoresis assay and PCR-Southern hybridization showed the foreignbar gene had been stably transformed into the genomes of the transgenic wheat plantletsregenerated from 4 of the 9 ppt-resistant calluses.

关 键 词：小麦 BAR基因 基因枪 转基因植株 

分 类 号：S512.103.2[农业科学—作物学] S188