应用高效液相色谱法测定母乳化奶粉中的牛磺酸  

DETERMINATION OF TAURINE IN HUMANIZED MILK POWDER BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY(HPLC)

作　　者：叶怀庄[1] 任一平 黄百芬 

机构地区：[1]浙江医科大学公共卫生学院卫生检测中心 [2]浙江省轻工业研究所

出　　处：《浙江医科大学学报》1997年第1期26-29,共4页

摘　　要：牛磺酸作为新型营养强化剂添加在第二代母乳化奶粉中。作者应用高效液相色谱法对母乳化奶粉中的牛磺酸进行定量分析，样品经1％偏磷酸提取→钠柱分离→柱后OPA衍生→荧光检测器检测定量。操作简便、快速，提取完全，牛磺酸出峰时间8．738min，回收率99．5％，变异系数2．19％，最低检出量20ng，为一个较理想的定量方法。Taurine,a new nutritive fortifier, has been added to humanized milk powder of the second generation. In this study taurine was determined with HPLC. The samples were extracted by 1%metaphosphoric acid,separated by Na+-column,derivatizated with OPA and detected by fluorescence detector. The retention time of taurine was 8. 738 min,the recovery rate being 99. 5 %,the variation coefficient(CV% )2. 19% and the minimum detectable quantity was 20 ng.

关 键 词：母乳化奶粉 牛磺酸 高效液相色谱 

分 类 号：R155.57[医药卫生—营养与食品卫生学]