日本曲霉菌体固定化及改性大豆低聚糖的研究  

Immobilization of aspergillus japonicus pellets and bioconversion of soybean oligosaccharide

作　　者：王立梅[1] 齐斌[1] 周惠明[2] 

机构地区：[1]常熟理工学院生物与食品工程系,江苏常熟215500 [2]江南大学食品学院,江苏无锡214036

出　　处：《食品与机械》2007年第1期15-17,24,共4页Food and Machinery

摘　　要：将日本曲霉经液体培养制备成1mm左右的菌球,以戊二醛为交联剂,牛血清蛋白为活性保护剂对其进行固定化,得到了果糖转移酶活力和稳定性均较高的固定化菌体细胞。利用固定化菌体细胞改性大豆低聚糖,蔗糖的转化率可达76%。The Aspergillus japonicus mycelium pellets with 1 mm diameter cultured in liquid were immobilized with glutaraldehyde and BSA. The fructosyhransferase in the immobilized mycelium pellets showed excellent activity and stability. The biocoversion of soybean oligosaccharides using the immobilized mycelium pellets was investigated. After treatment with immobilized pellets , 76% sucrose in soybean oligosaccharides was changed into other sugar.

关 键 词：日本曲霉 菌体 交联法 固定化 果糖转移酶 大豆低聚糖 

分 类 号：TS201.3[轻工技术与工程—食品科学]