抑癌基因PTEN突变在头颈部肿瘤中的作用  

Detection of PTEN mutations of in head and neck tumor

作　　者：王心蕊[1] 余子洋[2] 王苹[3] 郝黎明[1] 刘静秋 杜波[3] 

机构地区：[1]吉林大学人兽共患病教育部重点实验室,吉林长春130051 [2]吉林大学农学部门诊部 [3]吉林大学第一医院耳鼻咽喉-头颈外科 [4]吉林省产品质量监督检察院

出　　处：《中国实验诊断学》2007年第7期874-877,共4页Chinese Journal of Laboratory Diagnosis

基　　金：世界卫生组织(WHO)资助课题(H-16-133PBD)

摘　　要：目的头颈部肿瘤与PTEN基因的关系国外虽已有报道,但结论并不一致,本文通过对头颈部肿瘤患者进行PTEN基因突变的筛查及分析,力求发现PTEN基因突变与头颈部肿瘤发生发展中的作用。方法采用多聚酶链式反应和非放射性同位素标记的单链构象多态性分析(Non-RISSCP)技术,对头颈部肿瘤62例、喉乳头状瘤8例进行了PTEN抑癌基因9个外显子的突变分析,同时采用免疫组化方法检测PTEN蛋白在肿瘤组织和喉良性病变中的表达。结果SSCP分析发现,所有样本均未有异常的电泳带,没有基因突变被检测出。免疫组化结果显示,所有的肿瘤组织和喉良性病变均表达高水平的PTEN蛋白。结论我们推测PTEN基因突变在头颈部癌症的形成中可能不起主导作用。Objective Recently study demonstrate that PTEN gene is mutated in a wide range of human cancers, including glioblastoms,melanoma,and prostate, breast, and endometrial cancers. But, the correlation of head and neck tumor and PTEN gene mutation and expression are rarely reported.Methods We obtained DNA samples of 62 head and neck tumor and 8 laryngeal papillomas. Using PCR （polymerase chain reaction）- SSCP（single-strand conformation polymouphism）, we analyzed the coding region of PIEN gene for mutation. Meanwhile, tumor tissue were used for the immunohistochemical evaluation of PTEN expression. Results No mutation was detected. PTEN gene mutation dose not play a major role in head and neck tumor. Immtmohistochemical study showed that the PYEN/MMAC1 protein was localized dominantly in the cytoplasm and strongly stained in all 62 squamous cell carcinomas and laryngeal papillomas. Conclusion While many cancer genes are associated with head and neck tumorigenesis, our study suggests that PTEN is not among them.

关 键 词：头颈部肿瘤 PTEN 基因突变 抑癌基因 

分 类 号：R739.91[医药卫生—肿瘤]