离子交换法解除丁酸梭状芽孢杆菌发酵液中丁酸抑制的研究  

Study on Removing Inhibition of Butyric Acid from Clostridium Butyricum Fermentation Broth Using Ion-exchange Method

作　　者：史文玉[1] 路福平[1] 李涛[1] 田琳[1] 李斌[1] 杜连祥[1] 

机构地区：[1]天津市工业微生物重点实验室天津科技大学生物工程学院,天津300222

出　　处：《食品与发酵工业》2007年第6期13-15,共3页Food and Fermentation Industries

摘　　要：采用离子交换法吸附发酵液中的丁酸，从18种树脂中筛选出了1种对丁酸吸交性能较好的树脂A。树脂A对10g／L的丁酸溶液中丁酸的静态吸附量为243．3g／kg，对5g／L丁酸梭状芽孢杆菌发酵液中丁酸的静态吸附量则为142．2g／kg。动态吸附实验表明，树脂A吸附性能良好，洗脱峰高而窄，再生性能优良。5L发酵罐实验表明，发酵残液经陶瓷膜过滤，再经过离子交换树脂脱酸后，补充新鲜培养基打入发酵罐中进行循环使用，大大降低了原发酵液中丁酸对丁酸梭状芽孢杆菌生长的抑制，大幅度提高了菌体浓度，使之由补料分批高浓度培养时的6．1×10^8个／mL提高到1．2×10^9个／mL。ABSTRACT High-density culture must be carried out before the preparation of microecological agents. We used ion-exchange method to remove butyric acid from fermentation broth..An appropriate resin A was select- ed from more than 18 types of resins. The static state absorption capacity of Resin A was 243. 3 g/kg from 5g/L butyric acid solution and 142.2g/kg from 6g/L fermentation broth. The dynamic state exchange trials, including adsorption,elute and regeneration of the resin was performed. After removing of butyric acid from fermentation broth using ion-exchange method,we studied high cell density culture with fed-batch culture in bioreactor. Cell concentration of Clostridium butyricurn in5L bioreactor high-density culture increased from 6. 1×10^8 个/mL to 1.2×10^9 个/mL.

关 键 词：丁酸 丁酸梭状芽孢杆菌 抑制 树脂 

分 类 号：TQ920[轻工技术与工程—发酵工程]