Cysteinyl leukotriene receptor 1 partially mediates brain cryoinjury in mice  

作　　者：Qian DING San-hua FANG Yu ZHOU Li-hui ZHANG Wei-ping ZHANG Zhong CHEN Er-qing WEI 

机构地区：[1]Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou 310058, China [2]Department of Pharmacology, School of Medicine, Hangzhou Teachers College, Hangzhou 310012, China

出　　处：《Acta Pharmacologica Sinica》2007年第7期945-952,共8页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China(№ 30672449)

摘　　要：Aim： To determine whether the cysteinyl leukotriene receptor 1 （CysLT1 receptor） modulates brain cryoinjury and whether the CysLT1 receptor antagonist pranlukast exerts a time-dependent protective effect on cryoinjury in mice. Methods： Brain cryoinjury was induced by applying a liquid nitrogen-cooled metal probe to the surface of the skull for 30 s. Brain lesion, neuron density, and endogenous IgG exudation were observed 24 h after cryoinjury. Transcription and the expression of the CysLT1 receptor were detected by RT-PCR and immunoblotting, and the localization of the receptor protein by double immunofluorescence. Results： The mRNA and protein expressions of the CysLT1 receptor were upregulated in the brain 6-24 h after cryoinjury, and the CysLT1 receptor protein was primarily localized in the neurons, not in the astrocytes or microglia. Pre-injury treatments with multi-doses and a single dose of pranlukast （0.1 mg/kg） attenuated cryoinjury; postinjury single dose （0.1 mg/kg） at 30 rain （not 1 h） after cryoinjury was also effective. Conclusion： The CysLT1 receptor modulates cryoinjury in mice at least partly, and postinjury treatment with its antagonist pranlukast exerts the protective effect with a therapeutic window of 30 rain.Aim： To determine whether the cysteinyl leukotriene receptor 1 （CysLT1 receptor） modulates brain cryoinjury and whether the CysLT1 receptor antagonist pranlukast exerts a time-dependent protective effect on cryoinjury in mice. Methods： Brain cryoinjury was induced by applying a liquid nitrogen-cooled metal probe to the surface of the skull for 30 s. Brain lesion, neuron density, and endogenous IgG exudation were observed 24 h after cryoinjury. Transcription and the expression of the CysLT1 receptor were detected by RT-PCR and immunoblotting, and the localization of the receptor protein by double immunofluorescence. Results： The mRNA and protein expressions of the CysLT1 receptor were upregulated in the brain 6-24 h after cryoinjury, and the CysLT1 receptor protein was primarily localized in the neurons, not in the astrocytes or microglia. Pre-injury treatments with multi-doses and a single dose of pranlukast （0.1 mg/kg） attenuated cryoinjury; postinjury single dose （0.1 mg/kg） at 30 rain （not 1 h） after cryoinjury was also effective. Conclusion： The CysLT1 receptor modulates cryoinjury in mice at least partly, and postinjury treatment with its antagonist pranlukast exerts the protective effect with a therapeutic window of 30 rain.

关 键 词：cysteinyl leukotriene receptor ANTAGONIST PRANLUKAST CRYOINJURY NEUROPROTECTION 

分 类 号：R651.1[医药卫生—外科学]