雀形目15种鸟类CoⅠ与Cyt b基因序列的比较  

COMPARISON OF CYT B AND COⅠ GENE SEQUENCES FROM 15 SPECIES IN PASSERIFORMES

作　　者：梁刚[1] 张卫[1] 雷富民[2] 尹祚华[2] 黄原[1] 李天宪[3] 

机构地区：[1]陕西师范大学生命科学学院,西安710062 [2]中国科学院动物研究所,北京100080 [3]中国科学院武汉病毒所,武汉430071

出　　处：《动物分类学报》2007年第3期613-620,共8页Acta Zootaxonomica Sinica

基　　金：国家自然科学基金资助项目(30670276;30270203;30599431);中国科学院动物研究所知识创新领域前沿项目(KSCX3-IOZ-0614);NSFC/KOSEF协议项目(30611140611);陕西师范大学校级重点科研项目资助

摘　　要：对雀形目Passeriformes6科15种鸟类线粒体DNA的Cyt b基因全序列(1143bp)和CoⅠ基因部分序列(1176bp)进行了比较,结果显示Cyt b和CoⅠ基因序列的变异位点分别为454个和366个,简约信息位点为337个和303个,而且CoⅠ基因比Cytb基因略微保守,进化速率也较低。采用邻接法、最大简约法、最大似然法和贝叶斯法分别构建了CoⅠ和Cyt b基因两组数据集的分子系统发生树及其合一树,并对建树结果进行了比较分析。基于以上两点,本文认为CoⅠ基因比Cyt b基因更适合于确定雀形目科级阶元之间的系统发生关系,而且它也能够作为雀形目物种鉴定的分子标记,但在物种鉴定方面不如Cyt b基因稳定和准确。By using two molectflar markers of mitochondrial DNA, i. e. Cyt b and Co Ⅰ gene, the sequencess of 15 species representing 6 famifies from Passeriformes were compared. The results showed that the variable sites of Cyt b and CoⅠ gene sequence were 454 and 366, and parsimony information sites were 337 and 303 respectively. Moreover, CoⅠ gene was more conservative and had a lower evolutionary rate than Cyt b gene. Neighbor-joining（N J）, maximum-parsimony （MP）, maximum-likelihood （ML） and Bayesian （BI）analyses generated nearly identical tree topologies and their fifty percent consensus trees were computed. The results of these phylogenefic trees were compared and analyzed, too. It was also revealed that CoⅠ gene was more suitable to identify the phylogenetic relationship of avian family unit than Cyt b gene, and CoⅠ gene can be a molecular marker to identify avian species. But in species identification, Co Ⅰ gene was less stable and accurate than Cyt b gene.

关 键 词：雀形目 CoⅠ基因 CYT B基因 序列比较 

分 类 号：Q953[生物学—动物学]