孔雀石绿降解菌M3的分离鉴定及降解特性研究  

Isolation and Identification of Malachite Green-Degrading Bacteria M3 and Its Degradation Characteristics.

作　　者：任倩[1] 蒋丽娟[1] 宋炜[1] 杨柳燕[1] 肖琳[1] 张彤晴[2] 林海[2] 潘建林[2] 

机构地区：[1]南京大学环境学院/污染控制与资源化研究国家重点实验室,江苏南京210093 [2]江苏省淡水水产研究所,江苏南京210017

出　　处：《生态与农村环境学报》2007年第3期65-69,共5页Journal of Ecology and Rural Environment

基　　金：江苏省科技计划项目(BG2005324)

摘　　要：从鱼塘底泥中筛选分离出1株能高效降解低含量孔雀石绿(MG)的细菌M3。经16S rDNA同源性序列分析,鉴定为泛菌属(Pantoeasp.)。30℃静止培养条件下,该菌株对0.5、1.0、2.0和5.0 mg.L-1孔雀石绿5 d的降解率分别为97.54%、97.1%、100%和77.8%。菌株M3不能以MG为唯一碳源进行生长和代谢。葡萄糖、NH4NO3、KH2PO4/K2HPO4均能影响菌株M3对MG的降解。20～30℃温度范围内菌株M3对MG有明显降解效果,且降解速率随温度上升而提高。A strain of bacterium M3, capable of degrading malachite green efficiently, was screened out of sediment of a fish pond. Through 16S rDNA sequential analysis M3 was identified as Pantoea sp. When the concentration of malachite green was 0.5,1.0,2.0 and 5.0 mg·L ^-1, its degradation rate was 97.54%, 97.1%, 100 % and 77.8 %, respectively in 5 days of static incubation at 30℃. But the strain is unable to utilize malachite green as its sole carbon source. Glucose, NH4NO3 and KH2PO4/K2HPO4 can affect the degradation of malachite green. At the temperature ranging from 20 to 30℃, the strain can efficiently degrade malachite green with its degradation rate increasing with the temperature.

关 键 词：孔雀石绿 生物降解 16SrDNA 降解条件 

分 类 号：X172[环境科学与工程—环境科学] X131