α-葡萄糖苷酶高产菌株诱变选育的研究  

Screening of a Mutant Strain Producing Alpha-transglucosidase

作　　者：陈桂光[1] 管立忠[1] 李玮[1] 陈山岭[1] 梁智群[1] 

机构地区：[1]广西大学生命科学与技术学院,广西南宁530004

出　　处：《现代食品科技》2007年第7期19-21,共3页Modern Food Science and Technology

摘　　要：α-葡萄糖苷酶是生产异麦芽低聚糖的关键酶。本文以α-葡萄糖苷酶产生菌黑曲霉M-1为出发菌株,经UV、UV-LiCl复合诱变和60Co诱变选育,成功筛选出了一株遗传稳定性较好的高产菌株C-1,其产酶活力是出发菌株的2.7倍。有望将来应用于异麦芽低聚糖的生产中。Alpha-transglucosidase plays a critical role in producing isomalto oligosaccharide. In the paper, A stable mutant strain A.nigerC-I producing alpha-transglucosidase was obtained by ^60Co, UV and UV- LiCI mutant experiments. The productivity of the Alpha-transglucosidase of the mutagenesis is 2.7 times higher than that of the wild strain A.niger M-I.The strain would be applied in the production of isomaltooligosaccharide in the future.

关 键 词：Α-葡萄糖苷酶 ^60CO 诱变选育 

分 类 号：Q933[生物学—微生物学]