非小细胞肺癌组织中c-k-ras基因点突变的研究  

A STUDY OF THE c-k-ras ONCOGENE POINT MUTATION IN NON-SMALL CELL LUNG CANCER TISSUES

作　　者：谭永红[1] 张培炽[1] 钱桂生[2] 

机构地区：[1]第三军医大学大坪医院,630042 [2]第三军医大学附属新桥医院呼吸中心

出　　处：《重庆医学》1997年第1期3-4,共2页Chongqing medicine

摘　　要：作者采用聚合酶键反应和限制性片段长度多态分析方法，研究非小细胞肺癌组织中c－k－ras基因第12位密码子点突变情况。结果发现：29例被检测标本中5例有点突变，其发生频率为17．24％；相同病理分期，点突变阴性患者的临床生存期明显长于点突变阳性者。结果提示，c－k－ras基因点突变是独立于病理TNM分期的有意义的阴性预后指标。The c-k-ras oncogene point mutation at codon 12 in non-smaU cell lung cancer were investigated by polymerase chain reaction and restriction fragment length polymorphism analysis. The results revealed that aniong 29 tumor cases, 17. 24% of them were found to mutate at codon 12 of the c-k-ras oncogene. In the same pathologic stage,patients with normal k-ras twelfth codon survival significantly those with k-ras twelfth codon mutation. We conclused that the point mutation of c-k-ras oncogene twelfth codon is major prognostic marker and independent of pathologic stage

关 键 词：K-RAS基因 点突变 预后 肺肿瘤 

分 类 号：R734.2[医药卫生—肿瘤]