豆豉溶栓酶提取纯化研究  

STUDY ON EXTRACTION AND PURIFICATION OF DOUCHI FIBRINOLYTIC ENZYME

作　　者：姚晓玲[1] 孟凡涛[1] 吴思方[1] 

机构地区：[1]湖北工业大学生物工程学院,湖北武汉430068

出　　处：《食品研究与开发》2007年第8期79-82,共4页Food Research and Development

基　　金：武汉市重大攻关项目(2002600513422)

摘　　要：对豆豉溶栓酶发酵液进行提取、脱色,盐析,超滤脱盐,冷冻干燥等纯化研究,制得的豆豉溶栓酶干品,其比活力为530.6U/mg,纯化倍数为11.0,活性回收率为65.7%。溶栓酶干品经Sephadex G-100柱层析,SDS-PAGE法测定分子量为31.0ku。The extraction and purification through decolorizing, salting-out, desahing by uhrafihration and freezing-dry of Douchi fibrinolytic enzyme from fermenting liquor were investigated. The enzymatic activity reached 530.6 U/mg. The purification ratio was 11.0 more times than before, and the reclaim ratio was 65.7 %. Through sephadex-100 column chromatography, the molecular weight of dry Douchi fibrinolytic enzyme was 31.0 KD determined by SDA-PAGE.

关 键 词：豆豉溶栓酶 纯化 比活力 

分 类 号：TQ464.8[化学工程—制药化工]