高效液相色谱法分析三氯蔗糖的含量  

Determination of Sucralose by High Performance Liquid Chromatography

作　　者：周维义[1] 邓小娟[1] 

机构地区：[1]天津大学分析中心,天津300072

出　　处：《食品科学》2007年第7期360-361,共2页Food Science

摘　　要：目的:建立三氯蔗糖高效液相色谱测定方法。方法:采用DIKMAC18色谱柱(200mm×4.6mmi.d.5μm),流动相为甲醇-水(体积比为65:35),流速1.0ml/min,示差折光检测器,柱温40℃。结果:三氯蔗糖质量浓度在1.5～2000mg/L范围内与峰面积呈良好的线性关系,平均回收率为99.65%,相对标准偏差(RSD)为0.15%。结论:方法简便快速,操作稳定,经济环保,适用于三氯蔗糖的分析。Objective： A high performance liquid chromatographic method was developed to determine sucralose. Method： The column of DIKMA C18 （250 mm × 4.6 mm i.d. 5 μm） and a mobile phase of methanol-water （65：35, V/V） at a flow rate of 1.0 ml/min and column temperature 40 ℃ with refractive index detector were adopeted. Results： The method is proved to be linear in the range of sucralose from 1.5~2000 mg/L. The recovery is 99.65%. The relative standard deviation （RSD） is 0.15%. Conclusion： The method is simple, fast and stable. It is suitable for quantitative analysis for sucralose production process.

关 键 词：高效液相色谱 三氯蔗糖 示差检测器 

分 类 号：TS207.3[轻工技术与工程—食品科学]