利用电激法转化小麦幼胚的研究  

A STUDY ON GENE TRANSFORMATION OF IMMATURE WHEAT EMBRYOS BY ELECTROPORATION

作　　者：柯遐义[1] 黄粤 石和平[1] 李宝健[1] 

机构地区：[1]中山大学生物工程中心,广州510275

出　　处：《武汉植物学研究》1997年第2期103-107,共5页Journal of Wuhan Botanical Research

基　　金："八六三"高科技资助

摘　　要：利用我们实验室自制的脉冲放电式电激仪HPES－3，我们将合bar基因即PAT酶（phosphinothricinacetyltransferase）基因的pDM302质粒DNA导入了一个春小麦品种（TriticumaestivumL．）“T2003”的幼胚中。经过在含有ppt（phosphinothricin）的选择培养基上筛选，得到了抗ppt的愈伤组织和再生小苗。PCR检测与DNA点杂交结果显示，外源bar基因已整合进了转基因的小麦植株中。By use of the HPES-3 pulse discharging electroporation system that is developed by our laboratory, we have transferred the bar gene(phosphinothricin acetyltransferase gene) in plasmid pDM302 into the immature embryos of a spring wheat cultivar (Triticum aesivum L. ) 'T2003'. After selection for 2~ 3 months, we have obtained the ppt (phosphinothricin)-resistant callus, from which regenerated a wheat plantlet. PCR analysis and DNA dot hybridization showed the foreign bar gene had been integrated into the genomes of the transformed wheat plantlet.

关 键 词：小麦 幼胚 电激 转基因植株 基因工程 

分 类 号：S512.103.2[农业科学—作物学] S188