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机构地区:[1]烟台大学生化系,山东烟台264005 [2]华东师范大学生命科学学院,上海200062
出 处:《安徽农业科学》2007年第22期6799-6800,共2页Journal of Anhui Agricultural Sciences
基 金:上海市科技兴农重点攻关项目(农科技字(98)04-5号)
摘 要:[目的]初步探讨用50-DE微囊强化剂对褶皱臂尾轮虫进行强化培养,找出新的强化培养方案。[方法]用不同浓度的50-DE微囊脂肪酸强化剂对褶皱臂尾轮虫进行营养强化。[结果]轮虫浓度为250 ind/ml时,强化剂有效浓度为3×10^6-3×10^8ind/ml,最佳浓度为3×10^7ind/ml。强化最佳效应时间为12-16 h,强化48 h后强化组轮虫脂类含量仍高于对照组(酵母培养组)。[结论]强化剂浓度太高不仅不能有效提高轮虫的总脂含量,而且会导致被强化轮虫的密度下降,水体环境质量改变也与之有关。轮虫的生活需要洁净的水体,水体中含有过多的脂质强化剂微囊时,轮虫的活动空间以及水质都会受到不同程度的影响,从而影响轮虫的存活和生长。[ Objective ] The purpose was to discuss preliminarily the strengthening culture with 50-DE microcapsule strengthener to Branchionus plicatilis and find a new strengthening culture plan. [ Method] The strengthening nutrition culture was conducted with .50-DE microcapsule strengthener with different concentrations on B. plicatilis. [ Result] When the concentration of rotifer was 250 ind/ml, the effective concentration of strengthener was 3 ×10^6 - 3 ×10^8s ind/ml and the best concentration was 3 ×10^7 ind/ml. The best effect time of strengthening was 12 - 16 h, and after strengthening for48 h, the lipids content of strengthened rotifer was also higher than that of CK ( yeast culture group). [ Conclusion ] The strengthener with too high concentration not only couldn' t enhance the total ]ipids content of rotifer effect/vely, but also could cause the density of strengthened rotifer to decrease, which was related to the change of water-body environmental quality. The life of rotifer needed clean water-body. When the water-body contained too many lipid strengthener microcapsules, the active room of rotifer and water quality was influenced to different extents, so its survival and growth were influenced.
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