肺癌组织中Cyr61、WISP-1基因表达及意义  

Expression of Cyr61 and WISP-1 in human lung cancer tissues and their clinical significance

作　　者：崔玲玲[1] 王旗[1] 赵松[2] 赵高峰[2] 秦建军[2] 谢东[1] 陈萍萍[1] 

机构地区：[1]郑州大学公共卫生学院,河南郑州450052 [2]郑州大学第一附属医院

出　　处：《山东医药》2007年第20期1-3,共3页Shandong Medical Journal

基　　金：河南省创新人才基金项目(200400056);河南省教育厅自然科学研究指导计划(2006330004);河南省国际合作计划项目(074300510001)

摘　　要：目的研究人肺癌组织中Cyr61和WISP-1的基因表达水平及其与临床参数的关系。方法采用real-time PCR和免疫组织化学染色法对60例肺癌患者的肺癌组织和癌旁正常肺组织Cyr61和WISP-1表达水平进行测定。结果癌组织中Cyr61表达水平低于癌旁正常肺组织,WISP-1表达水平高于癌旁组织(P均<0.05);Cyr61水平与病理类型、病理分级、转移、吸烟及家族史等相关(P均<0.05),WISP-1与病理类型和年龄相关(P均<0.05)。结论Cyr61和WISP-1在肺癌的发展中起重要作用;其表达的蛋白产物可作为临床早期诊断和干预治疗的重要指标。[Objective] To investigate Cyr61 and WISP-1 expression levels and their relations to clinical and pathological features in human lung carcinomas.[Methods]We quantified Cyr61 and WISP-1 expression levels in sixty lung cancer tissues and their matched normal lung tissues by using real-time PCR assay and immunohistochemistry staining.[Results]Down-regulation of Cyr61 and up-regualtion of WISP-1 were both found in lung cancers compared to the paired normal lung tissues（P〈0.05）.Cyr61 expression levels correlated with tumor grade,metastasis,pathology type,smoking and family history（P〈0.05）.Also,WISP-1 correlated with pathology type and age（P〈0.05）.[Conclusion]Cyr61 and WISP-1 may play important roles in the progression of lung cancer,and their proteins might serve as valuable targets for early diagnosis and therapeutic intervention.

关 键 词：CYR61 WISP-1 肺癌 REAL-TIMEPCR 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]