新型人趋化素样因子超家族8对人白血病细胞增殖和表皮生长因子受体表达的影响  

Effects of Novel Human Chemokine-like Factor Superfamily 8 on Proliferation and EGFR Expression of HL-60 Cells

作　　者：陈绍倩[1] 杜英[2] 黄玉敏[2] 王鑫[3] 顾巧丽[3] 董子明[3] 

机构地区：[1]郑州大学第一附属医院血液科,郑州450055 [2]郑州大学基础医学院免疫学教研室,郑州450042 [3]郑州大学基础医学院病理生理学教研室,郑州450042

出　　处：《中国实验血液学杂志》2007年第3期458-461,共4页Journal of Experimental Hematology

摘　　要：本研究探讨新型趋化因子CKLFSF8对人白血病HL-60细胞的增殖和表皮生长因子受体(EGFR)表达的影响,应用RT-PCR方法检测CKLFSF8基因在HL-60细胞中的表达;利用脂质体将CKLFSF8基因转染到HL-60细胞,在倒置显微镜下观察细胞生长;MTT法检测细胞的增殖程度;免疫细胞化学法检测CKLFSF8基因转染前后HL-60细胞EGFR的表达。结果表明:在细胞生长过程中可检测到CKLFSF8表达;CKLFSF8基因转染前后HL-60细胞的增殖受到了明显的抑制,与空白对照组和空质粒组相比有显著性差异(P(0.05);对比转染前后细胞EGFR表达的阳性率具有显著性差异(P(0.01)。结论:CKLFSF8基因转染对白血病细胞HL-60的增殖和EGFR的表达有明显抑制作用。This study was aimed to investigate the effect of chemokine-like factor superfamily 8 （ CKLFSF8 ） on proliferation and expression of epidermal growth factor receptor （EGFR） of HL-60 cells. Expression of CKLFSF8 mRNA on HL-60 cell line was assayed by RT-PCR; the target gene was transfected into the cells by lipid vector, cell proliferation was determined by MTT assay, while expression of EGFR in HL-60 was determined by immunocytochemical technique. The results indicated that expression of CKLFSF8 existed in HL-60 cells. After transfection, cell proliferation was inhibited （ P 〈 0. 05 ） and the expression of EGFR in HL-60 cells was also discovered to be inhibited （ P 〈 0.05 ）. It is concluded that the proliferation and expression of EGFR in HL-60 cells can be inhibited by transfection of CKLFSF8. The novel chemokine may provide a new approach in the treatment of leukemia.

关 键 词：CKLFSF8 HL-60细胞 细胞增殖 EGFR 

分 类 号：R733.7[医药卫生—肿瘤] R329.28[医药卫生—临床医学]