基因组改组技术快速提高扩展青霉碱性脂肪酶产量  

Rapid Improvement of Lipase Production in Penicillium expansum by Genome Shuffling

作　　者：林峻[1] 施碧红[1] 施巧琴[1] 何云霞[1] 王明兹[1] 

机构地区：[1]福建师范大学教育部工业微生物工程研究中心,福州350018

出　　处：《生物工程学报》2007年第4期672-676,共5页Chinese Journal of Biotechnology

基　　金：福建省自然科学基金资助(No2006J0073);福建省青年人才创新基金(No2004J013);福建省教育厅A类项目(NoJA050200)~~

摘　　要：应用基因组改组技术快速提高扩展青霉碱性脂肪酶的产量。采用经过多代诱变的碱性脂肪酶产生菌扩展青霉(Penicillium expansum)FS8486以及分离自新疆火焰山口土样的溜曲霉(Aspergillus tamarii)FS-132作为出发菌株,经过两轮基因组改组,得到数株优良子代。其中一株酶活较出发菌株FS8486提高317%。对亲本与子代菌株的形态型、RAPD(随机扩增多态性DNA)多态性和脂肪酸组成分析初步确定筛选获得的菌株为亲本的改组子代。首次将基因组改组技术成功应用于真核微生物基因组改造,短期内使目标代谢产物获得提高,这对于在真核微生物育种中进一步推广该技术具有重要意义。In the present study, the genome shuffling was used to improve lipase production of PeniciUium expansum. A lipase producing mutant strain-Penicillium expansum FS8486 and a wild type of Aspergillus Tamarii FS-132 isolated from soil of a volcano in Xinjiang were used as the parental strains. After two rounds of genome shuffling, several elite daughter strains were screened. The lipase activity in one of the daughter strains was increased 317 % over the starting strain FS8486. Comparisons of the morphology, RAPD （Random Amplification of Polymorphic DNA） polymorphism and the fatty acid compositions between the daughter and the parental strains suggested that the filial generation were generated by genome shuffling. In this study, the genome shuffling used successfully first time in eukaryotic microorganism and increases the production of the desired metabolite in short time, the study will be useful to spread the genome shuffling in eukaryotic microbial breeding.

关 键 词：基因组改组 扩展青霉 碱性脂肪酶 溜曲霉 

分 类 号：Q789[生物学—分子生物学]