表没食子儿茶素没食子酸酯诱导人肝癌SMMC-7721细胞凋亡的实验研究  

Induction of Apoptosis by Epigallocatechin-3-gallate in Liver Cancer Cell Line SMMC-7721

作　　者：王宇[1] 韩丽娟[2] 

机构地区：[1]长治医学院病理学教研室,046000 [2]长治医学院附属和平医院生殖中心

出　　处：《长治医学院学报》2007年第4期241-244,共4页Journal of Changzhi Medical College

摘　　要：目的:观察表没食子儿茶素没食子酸酯(EGCG)诱导人肝癌(SMMC-7721)细胞系,细胞凋亡的生物学效应。方法:通过MTT比色法检测EGCG对SMMC-7721细胞生长的影响;应用细胞计数法研究不同浓度EGCG对SMMC-7721细胞生长曲线的影响;PI染色流式细胞术研究EGCG对SMMC-7721细胞周期和凋亡率的影响。结果:EGCG显著抑制人肝癌SMMC-7721细胞的生长,呈浓度依赖性;SMMC-7721细胞经EGCG作用后,其细胞群体倍增时间明显延长,细胞增殖周期延长,从而细胞增殖速度减慢;流式细胞术分析结果表明EGCG(30μg/mL、60μg/mL、120μg/mL)处理SMMC-7721细胞48h后,G1期细胞累积均逐渐增加。结论:EGCG具有诱导人肝癌SMMC-7721细胞凋亡的作用,其机制可能与使细胞周期G1期阻滞相关。Objective：To investigate the effect of epigallocatechin - 3 - gallate on induction of apoptosis in liver cancer cell line SMMC - 7721. Methods：The growth inhibition of SMMC - 7721 cells was measured by MTT assay. Cell couting method was used to test the influence of growth curve in SMMC - 7721 cells treated with different concentrations of EGCG. The apoptosis rate and cell cyle of SMMC - 7721 cells were determined by PI staining flow cytometry. Results： EGCG significantly inhibited the growth of SMMC- 7721 cells,in a concentration dependent manner. Cell counting showed that average doubling time increased by EGCG in a concentration dependent manner. PI staining flow cytometry showed that cell cyle was arrested at G1 phase and the apoptosis of SMMC -7721 cells was induced by EGCG in a concentration dependent manner. Conclusion：EGCG posseses antitumor effect in vitro,its mechanism might be associated with G1 phase cell cycle arrest.

关 键 词：肝肿瘤 表没食子儿茶素没食子酸酯 细胞周期 凋亡 

分 类 号：R361.3[医药卫生—病理学]