利用cDNA-AFLP技术研究茶树花蕾发育基因差异表达片段  

Analysis of Differential Gene Expression of Flower bud of Tea Plant (Camellia sinensis) by cDNA-AFLP

作　　者：余梅[1] 江昌俊[1] 叶爱华[1] 王朝霞[1] 朱林[1] 邓威威[1] 高轩[1] 宛晓春[1] 

机构地区：[1]安徽农业大学茶叶生物化学和生物技术教育部,农业部重点实验室,安徽合肥230036

出　　处：《茶叶科学》2007年第3期259-264,共6页Journal of Tea Science

基　　金：安徽省自然科学基金项目(050410102)

摘　　要：利用cDNA-AFLP技术,对茶树花蕾发育期的基因表达进行了分析。结果表明,茶树花蕾在发育早期和晚期基因的表达中有明显差异,共显示1110条带,早期发育花蕾和晚期发育花蕾显示的特异条带分别为35条和87条;选取特异表达的晚期发育花蕾重复性较好的15个TDFs(Transcript Derived Fragment)在GenBank进行序列分析,结果显示,9个分别与氧化还原酶、染色体13、14-3-3蛋白、Amyrel基因、钙网蛋白、ATP硫化酶、接触反应/铁离子结合蛋白及花粉特异蛋白的序列有相似性;2个与假拟蛋白相似,4个在GenBank数据库中未找到相似序列。For studies of differential gene expression, cDNA-AFLP was analyzed in tea plant at the stage of bud flower development. The result showed that there were evident differential expression in flower between the early stage and the late stage. Of the 1110 fragments inspected, 35 and 87 specially displayed in the early and late stages; 15 fragments were cloned and sequenced. Sequence analysis indicated that among 15 squenced fragments, nine were sequence-similar to proteins in GenBank, i.e. oxidoreductase, chromosome 13, 14-3-3 protein, Amyrel gene, calreticulin interacted protein, ATP sulfurylase, catalytic / iron ion binding, desiccation-related protein and ntp302, two were similar to hypothetical proteins, and no similar sequence were found in GenBank for the other 4 fragment.

关 键 词：花蕾发育 cDNA—AFLP 茶树 基因差异表达 

分 类 号：S571.1[农业科学—茶叶生产加工] Q523[农业科学—作物学]