Treatment of Scrapie Pathogen 263K With Tetracycline Partially Abolishes Protease-resistant Activity in vitro and Reduces Infectivity in vivo  

作　　者：YAN-JUN GUO JUN HAN HAI-LAN YAO BAO-YUN ZHANG JIAN-MEI GAO JIN ZHANG XIN-LI XIAO XIAO-FAN WANG WEI-QIN ZHAO DE-XIN WANG XIAO-PING DONG 

机构地区：[1]State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China [2]Department of Neurology, Beijing Friendship Hospital,Capital Medical University, Beijing 100050, China

出　　处：《Biomedical and Environmental Sciences》2007年第3期198-202,共5页生物医学与环境科学（英文版）

基　　金：This work was supported by National Natural Science Foundation of China,No.30130070;National 863 Project,No.2001AA215391;National Science and Technology Task Force Project,No.2001AA215391;EU Project QLRT,No.2000 01441.

摘　　要：Objective To study the possible effect of tetracycline on protease-resistant activity in vitro and infectivity in vivo of a scrapie strain 263K. Methods Scrapie pathogens were incubated with tetracycline at different concentrations for various periods of time and protease-resistant PrP signals were evaluated with proteinase K-treatment and Western blots. The preparations treated with tetracycline were intracerebrally inoculated into golden hamsters and typical TSE manifestations were noted. PrP^5c in brain tissues of the infected animals was detected by PrP specific Western blot assays. Results Protease-resistant PrP was significantly reduced in or removed from the preparations treated with tetracycline in a dose-dependant manner. Compared with the control group after incubated for 53.75±0.50 days, the preparations treated with 5 mmol/L and 20 mmol/L tetracycline prolonged the incubation time of 61.5±1.73 and 59.5±0.58 days （P〈0.05）. Conclusion Treatment of scrapie pathogen 263K with tetracycline reduces or removes its protease-resistant activity in vitro.Objective To study the possible effect of tetracycline on protease-resistant activity in vitro and infectivity in vivo of a scrapie strain 263K. Methods Scrapie pathogens were incubated with tetracycline at different concentrations for various periods of time and protease-resistant PrP signals were evaluated with proteinase K-treatment and Western blots. The preparations treated with tetracycline were intracerebrally inoculated into golden hamsters and typical TSE manifestations were noted. PrP^5c in brain tissues of the infected animals was detected by PrP specific Western blot assays. Results Protease-resistant PrP was significantly reduced in or removed from the preparations treated with tetracycline in a dose-dependant manner. Compared with the control group after incubated for 53.75±0.50 days, the preparations treated with 5 mmol/L and 20 mmol/L tetracycline prolonged the incubation time of 61.5±1.73 and 59.5±0.58 days （P〈0.05）. Conclusion Treatment of scrapie pathogen 263K with tetracycline reduces or removes its protease-resistant activity in vitro.

关 键 词：SCRAPIE PRION TETRACYCLINE Protease-resistant activity Animal experiment 

分 类 号：S858.26[农业科学—临床兽医学]