用镭射激光捕获技术研究LEC鼠p53基因突变和肝癌发生的关系  

Study of the relationship between p53 mutation of LEC rats and hepatocarcinogenesis by laser capture microdissection

作　　者：梁彬[1,2] 程大也[3] 梁红[4] 解方[1] 李丰[5] 

机构地区：[1]中国医科大学高等职业技术学院检验教研室 [2]中国医科大学卫生部细胞生物学重点实验室,辽宁沈阳110001 [3]中国医科大学附属第一医院输血科 [4]中国医科大学肿瘤研究所 [5]中国医科大学卫生部细胞生物学重点实验室

出　　处：《中华肿瘤防治杂志》2007年第16期1207-1209,1246,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：教育部博士点基金(20050159023)

摘　　要：目的:研究p53基因突变与原发性肝癌发生发展的关系。方法:利用LEC鼠(long evan cin-namon)作为肝癌的动物模型。选取15、37、101周的LEC鼠17、15和12只,正常对照SD大鼠15只,取出肝脏组织进行切片。切片一部分用于谷胱甘肽S-转移酶(GST-P)免疫组化染色,另一部分利用镭射激光捕获技术(laser capture microdissection,LCM)定位切割,进行p53基因外显子5、6/7、8突变的分析。结果:37周龄LEC鼠和101周龄LEC鼠GST-P阳性率高于对照组(P<0.05),15周龄LEC鼠GST-P阳性率与对照组相比差异无统计学意义,P>0.05。p53基因在GST-P表达阳性肝炎组有3例突变(3/12),GST-P表达阳性肝癌组有12例发生突变(12/17),肝癌组显著高于肝炎组,P<0.05。结论:p53突变发生在肝癌形成的早期阶段,随着发展过程,突变率明显增高。GST-P虽然特异性不强,但是不失为一种灵敏的判断肝损伤的指标。OBJECTIVE： To investigate the relationship between p53 mutation and primary hepatocarcinogenesis. METHODS： With LEC rats as animal model, 15 weeks LEC rats （17）, 37 weeks LEC rats （15） and 101 weeks LEC rats （12） were selected as the experiment group, 15 SD rats as the control. Some sections of the liver of the rats were used for immunohistochemical staining, the others were used for analysis of p53 mutation by LCM. RESULTS： Compared with the controls, GST-P expression ratios among 37 weeks and 101 weeks LEC rats were all higher （P〈0. 005）, but 15 weeks LEC rats were not （P〉 0. 05）. The ratio of p53 mutation in thehepatoma group with GST-P positive（12/17）was higher than that in the hepatitis group （ 3/12）, P 〈 0.05. CONCLUSIONS： p53 mutation runs through the initiative and late stages of hepato-carcinogenesis. With the progression, mutation ratio increases. Although GST-P specificity is not better,GST-P is a sensitive marker to define hepatic injury.

关 键 词：肝肿瘤 实验性/遗传学 多态现象 单链构象 基因 p53 突变 

分 类 号：R735.7[医药卫生—肿瘤]