聚-ε-赖氨酸高产菌株的筛选及其发酵工艺的初步研究  

Screening of Poly-ε-lysine-producing Strain and its Fermentation Technology

作　　者：陈雄[1] 章莹[1] 袁金凤[1] 王实玉[1] 王金华[1] 

机构地区：[1]湖北工业大学生物工程学院湖北省工业微生物重点实验室,武昌430068

出　　处：《微生物学通报》2007年第4期731-734,共4页Microbiology China

基　　金：湖北省教育厅青年项目(No.Q200514001)

摘　　要：利用亚甲基蓝平板初筛、摇瓶复筛,从土壤中筛选了一株聚-ε-赖氨酸(ε-PL)高产菌株,ε-PL摇瓶产量大于2g/L,经初步鉴定该菌株为白色链霉菌(Streptomyces albulus)。对该菌株发酵生产ε-PL的培养基成分进行初步研究表明:葡萄糖是最好的碳源,酵母粉和硫酸铵是最佳的复合氮源,在优化培养基下,ε-PL摇瓶产量达到3.9g/L。A strain with 2 g/L yield of poly-ε-lysine was firstly screened from soil on an agar plate containing methylene blue and then further selected by a flask experiment. The strain was preliminarily identified as Streptorayces albulus. The experiment about medium optimization showed that glucose was the best carbon sources and yeast extract and （NH4）2 SO4 were the best compound nitrogen sources. The yield of ε-PL reached 3.9 g/L with optimal medium

关 键 词：聚-ε-赖氨酸 白色链霉菌 筛选 发酵工艺 

分 类 号：TQ922.3[轻工技术与工程—发酵工程]