Fermentation Kinetics of Media Optimization for the Production of Alpha Amylase by a New Isolate of Aspergillus Oryzae  

作　　者：Ikram-ul-Haq Roheena Abdullah Hamid Mukhtar Muhammad Nauman Aftab 

机构地区：[1]Institute of Industrial Biotechnology, Government College University [2]Bioinformatics Laboratory, Institute of Biophysics, Chinese Academy of Sciences

出　　处：《微生物学通报》2007年第4期740-744,共5页Microbiology China

摘　　要：The present study is concerned with the isolation and screening of different strains of Aspergillus oryzae for the production of alpha amylase. Ninety strains were isolated from soil and tested for the production of alpha amylase in shake flasks. Of all the strains tested, Aspergillus oryzae GCB-32 and Aspergillus oryzae GCB-35 gave maximum production of alpha amylase. Different culture media were screened for the production of alpha amylase by these two strains. M1 medium containing starch, yeast extract, NH4Cl, MgSO4·7H2O and CaCl2 gave the maximum production of alpha amylase by both the strains Aspergillus oryzae GCB-32 and Aspergillus oryzae GCB-35.Kinetic analysis revealed that the values of product yield coefficient（Y p/x ） and specific product yield coefficient（qp） were found highly significant（p≤0.05） when medium M1 was used for the enzyme production.The present study is concerned with the isolation and screening of different strains of Aspergillus otyzae for the production of alpha amylase. Ninety strains were isolated from soil and tested for the production of alpha amylase in shake flasks. Of all the strains tested, Aspergillus otyzae GCB-32 and Aspergillus otyzae GCB-35 gave maximum production of alpha amylase. Different culture media were screened for the production of alpha amylase by these two strains. M1 medium containing starch, yeast extract, NH4Cl, MgSO4 ·7H2O and CaCl2 gave the maximum production of alpha amylase by both the strains Aspergillus oryzae GCB-32 and Aspergillus otyzae GCB-35. Kinetic analysis revealed that the values of product yield coefficient （ Yp/x ） and specific product yield coefficient （qp） were found highly significant （p ≤ 0.05 ） when medium M1 was used for the enzyme production.

关 键 词：发酵动力学 曲霉菌 Α淀粉酶 隔离种 最优化 媒介 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]