一种手性中间体在键合型和涂覆型纤维素手性固定相上的拆分  

Enantioselective separation of an intermediate enantiomer using immobilized and coated cellulose chiral stationary phases

作　　者：明永飞[1] 杨薇薇[2] 赵亮[1] 张红丽[1] 师彦平[1] 李永民[1] 

机构地区：[1]中科院兰州化学物理研究所甘肃省天然药物重点实验室 [2]西安市第四人民医院,西安710000

出　　处：《分析试验室》2007年第8期14-17,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20375045);(20675085);甘肃省自然科学基金(3ZS041-A25-23);中科院"西部之光"(2003)项目资助

摘　　要：制备了涂覆型和键合型纤维素-(3,5-二甲基苯基氨基甲酸酯)固定相,分别在制备的纤维素手性固定相上成功地拆分了一种手性中间体,通过考察流动相中的改性剂(醇、四氢呋喃、三氯甲烷)对手性拆分的影响,优化了手性中间体在两种手性固定相上的色谱分离条件,并比较了手性中间体在涂覆和键合型纤维素手性固定相上的拆分。结果表明,涂覆型和键合型手性固定相对这种手性中间体均有较好的拆分效果,在150 mm的色谱柱上,这两种手性固定相对这种手性中间体的拆分能力相差不大,但键合型固定相上可选择的流动相范围更广。Chiral stationary phases of immobilized and coated cellulose-3,5-dimethylphenyl-carbamates were prepared. The enantiomers of intermediate were resolved on the immobilized and coated chiral stationary phase, and the influence of modifiers （alcohols, THF and chloroform） in the mobile phase was investigated and the chromatographic conditions were optimized. The enantioselective separation of intermediate enantiomers on immobilized and coated cellulose chiral stationary phases was compared. The results showed that both immobilized and coated cellulose chiral stationary phase exhibited good stereoselectivity to the investigated intermediate and the ability of separation has no obvious discrepancy. However, the choice of mobile phase is broadened using immobilized cellulose stationary.

关 键 词：手性固定相 手性分离 键合 涂覆 手性中间体 

分 类 号：O656.3[理学—分析化学]