叔丁醇体系脂肪酶催化菜籽油制备生物柴油  

Preparation of bio-diesel from rapeseed oil catalyzed by lipase in t-BuOH

作　　者：刘志强[1] 李堂[2] 黄朋[2] 周建红[1] 

机构地区：[1]湖南科技大学生命科学学院,湘潭411201 [2]湖南科技大学化学化工学院,湘潭411201

出　　处：《农业工程学报》2007年第7期174-177,共4页Transactions of the Chinese Society of Agricultural Engineering

基　　金：973资助项目(2003CB716004);湖南省自然科学基金资助项目(02JJY5001)

摘　　要：为了在生物柴油制备中改善酶的催化环境,该文通过对脂肪酶LVK催化菜籽油乙醇解反应几个主要影响因素的研究,得出其最佳反应条件为:醇油摩尔比5∶1,催化剂用量10%,叔丁醇用量10%,反应温度40℃,反应时间为30 h,转酯率达到94.7%。研究表明10%的叔丁醇体系能完全解除乙醇对脂肪酶LVK的催化抑制作用;还消解除副产物甘油对脂肪酶LVK的失活。To improve catalytical environment of lipase, factors influencing rapeseed oil ethanolysis reaction catalyzed by lipase LVK were investigated. And the optimal reaction conditions were attained as follows. 5 ： 1 of ethanol/oil molar ratio, 10% enzyme and 10% t-BuOH based on oil weight, reaction at 40℃ for 30 h, under which the maximum ethyl esters yield can reach 94. 7%. The experimental results showed that t-BuOH（10%） could relieve the inhibition of ethanol on lipase LVK completely in the reaction system, and relieve deactivation of by-product glycerol on lipase LVK.

关 键 词：脂肪酶 菜籽油 乙醇解 生物柴油 

分 类 号：S565.4[农业科学—作物学]