微胶囊化儿茶素对阿霉素肾病大鼠VEGF表达的影响  

Effect of microcapsulated catechin on expression of vascular endothelial growth factor in rats with adriamycin induced-nephrotic syndrome

作　　者：何小解[1] 易著文[1] 杨华彬[1] 白海涛[1] 莫双红[1] 党西强[1] 

机构地区：[1]中南大学湘雅二医院小儿肾脏病研究室湖南省小儿肾脏病临床中心,湖南长沙410011

出　　处：《中国病理生理杂志》2007年第8期1570-1573,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30500546);湖南省自然科学基金资助项目(No.02JJXY3020);湖南省教育厅重点项目基金资助(No.02A015)

摘　　要：目的:研究微胶囊化儿茶素对阿霉素肾病大鼠血管内皮生长因子(VEGF)表达的影响。方法:将120只雌性SD大鼠随机分为对照组、肾病组、地塞米松组、维生素E组、儿茶素组和微胶囊组共6组,尾静脉1次性注射阿霉素(5 mg/kg BW)制备肾病模型;利用酶联免疫吸附试验测定血清与尿中VEGF含量;利用免疫组织化学方法检测肾组织中VEGF表达。结果:实验第4周末(维生素E组除外)与第6周末,各组大鼠尿、血清及肾组织中VEGF含量均显著高于对照大鼠、显著低于肾病大鼠(均P<0.01),实验末,微胶囊组大鼠尿与血清中VEGF含量明显低于儿茶素组(分别P<0.01,P<0.05),微囊组大鼠肾组织VEGF表达低于儿茶素组,但无显著差异。微囊化儿茶素治疗组24 h尿蛋白排泄量显著低于儿茶素治疗组(P<0.05),24 h尿蛋白排泄与尿、血清及肾组织中VEGF含量均显著正相关(P<0.01)。结论:儿茶素降低阿霉素肾病大鼠尿蛋白排泄可能是通过降低VEGF的排泄与分泌实现的,儿茶素微囊化后,有助于提高其降低VEGF的排泄与分泌,减少尿蛋白的排泄。AIM： To study the effect of microcapsulated catechin on vascular endothelial grower factor （VEGF） expression in rats with adriamycin induced -nephrotic syndrome. METHODS： 120 female SD rats were randomly distributed in control group, nephrotic group, dexamethason group, vitamin E group, catechin group and microcapsule group. Rat with nephrotic syndrome were induced by injection of adriblastine （5 mg/kg BW）. VEGF concentrations in serum and urine were detected by ELISA assay. VEGF expression in kidney was measured by immunohistochemistry assay. RESULTS： At the end of 4th week and 6th week, VEGF concentration in other .groups in kidney, serum and urine were higher than that in control （ all P 〈 0. 01 ）, and were lower than that in nephritic group （ exclude Vit E group, all P 〈 0. 01 ）. Serum and urine VEGF concentrations in microcapsule group were lower than those in catechin group （ P 〈 0. 01, P 〈 0. 05, respectively）. VEGF expression in microcapsule group in kidney was lower than that in catechin group, but it was not significant different. Urinary protein excretion at 24 h in microcapsule group was lower than that in catechin group （ P 〈 0. 05 ）, there was positive correlation among urine protein, serum VEGF level, urine VEGF concentration and renal VEGF expression at 24 h （ all P 〈 0. 01 ）. CONCLUSION ： Catechin decreases urinary protein excretion in the rats with nephrotic syndrome through reducing the expression and secretion of VEGF. Microcapusulated catechin is benefit in decreasing the expression and secretion of VEGF.

关 键 词：儿茶素 肾病综合征 内皮生长因子 大鼠 

分 类 号：R363[医药卫生—病理学]