胶体金免疫层析法半定量检测鸡肉中磺胺嘧啶残留  

Semi-quantitative Detection of Sulfadiazine by Immunochromatographic Assay in Chicken Meats

作　　者：王喜亮[1] 易俊东[2] 金秀娥[2] 李奎[1] 卢芳[2] 熊宁[1] 刘群[2] 刘百红[1] 毕丁仁[1] 

机构地区：[1]华中农业大学动物医学院,湖北武汉430070 [2]湖北省兽药监察所,湖北武汉430064

出　　处：《食品科学》2007年第8期337-340,共4页Food Science

基　　金：武汉市科技攻关计划项目(20022002062)

摘　　要：本研究采用柠檬酸三钠还原法制备胶体金,用胶体金标记磺胺嘧啶单克隆抗体,磺胺嘧啶竞争物包被于硝酸纤维素膜,制成免疫层析快速检测试纸条。该试纸条可以在15min内完成对磺胺嘧啶残留的半定量检测,灵敏度为5ng/ml,与其它磺胺类药物无交叉反应,在鸡肉中的回收率为71%～95%;用试纸条与高效液相法对来自动物试验的56份鸡肉样品进行对比检测。以我国磺胺嘧啶残留限量标准(100ng/g)为标准,二者符合率为100%。该方法灵敏度高,简便快速,无需特殊仪器设备,有良好的开发应用前景。Colloidal gold was prepared with reducing reaction of sodium citrate and was coulped with monocolonal anti- Sulfadiazine in this article. BSA-sulfadiazine was coupled with the nitrocellulose membranes to prepare immunochromatographic strips. With this method, semi-quantitative detection of sulfadiazine can be fmished in less than 15 min. The sensitivity to sulfadiazine is 5 ng/ml, and no cross-reactivity with other sulfonamides. Good recovery rates are determined from 71% to 95% in chickens meats. Based on maximum residue level of sulfadiazine （ 100 ng/g） in China, this method was compared with with high- performance liquid chromatographic （HPLC） by assaying 56 chicken meat samples taken from animal experiment. The agree- ment rates are 100%. This method is a sensitive, simple, rapid assay without the need of special apparatus, so it is of good practical value.

关 键 词：胶体金 免疫层析 鸡肉 磺胺嘧啶 

分 类 号：O658.1[理学—分析化学]