旋转生物传感器高灵敏检测盐酸克伦特罗方法研究  

Application of Immuno-rotary Biosensor Based on FoF1-ATPase in Chromatophores for Detecting Clenbuterol

作　　者：吕会田[1] 张云[2] 乐加昌[2] 张仲伦[2] 

机构地区：[1]北京工商大学化学与环境工程学院,北京100037 [2]中国科学院生物物理研究所,北京100101

出　　处：《食品科学》2007年第8期446-450,共5页Food Science

摘　　要：盐酸克伦特罗(clenbuterol hydrochloride, CL),又名双氯醇胺、氨哮素、克喘素,为白色结晶状粉末, 无味略苦,是一种人工合成的β2-肾上腺素受体激动剂。盐酸克伦特罗又称"瘦肉精",被非法用于肉用动物生产。彻底查禁CL 的非法滥用,必须要有高效、灵敏、特异的残留检测方法。本研究着重应用免疫学和生物化学方法,构建了基于量子点标记的ATP合酶分子马达免疫旋转生物传感器 (immuno-rotary biosensor, IRB),结合荧光技术,利用中国科学院生物物理研究所张仲伦老师研发的BPCL弱光检测器实现了对盐酸克伦特罗快速、高灵敏度的检测。Clenbuterol is a β-agonist used as a therapeutic agent in medicine. At high concentrations clenbuterol promotes the growth of farm animals but its use as a growth promoter is prohibited and its misuse has been related to food poisoning as results of consuming contaminated meat. Traditional methods for detection of clenbuterol often require elaborate sample preparation procedures and involve tedious time consuming protocols. So developing a new immuno-biosensor method for capture and detection such small molecular is still a big challenge. This research constructed an immuno-rotary biosensor （IRB） for clenbuterol detecting, which based on the fluorescent technique and BPCL （bilogical and chemical photon counter luminescene）. The results showed that a hypersensitive biosensor for quickly detection of clenbuterol was achieved.

关 键 词：盐酸克伦特罗 量子点 酶联免疫吸附测定 ATP合酶 免疫旋转生物传感器 BPCL 

分 类 号：TS207.5[轻工技术与工程—食品科学]