节律基因mPER1对小鼠Lewis肺癌细胞迁移的影响  

Influence of mPeriod1 on cell migration in Lewis lung carcinoma cells

作　　者：赵京卉[1] 汪宇辉[1] 刘延友[1] 段志青[1] 林丽[1] 要然[1] 朱小云[1] 王正荣[1] 

机构地区：[1]四川大学基础与法医学院卫生部时间生物学重点实验室,四川成都610041

出　　处：《西部医学》2007年第5期761-762,766,共3页Medical Journal of West China

摘　　要：目的通过节律基因mPeriod1(mPer1)过表达,研究mPer1对小鼠Lewis肺癌细胞迁移的影响。方法采用脂质体介导法将mPer1基因转染入Lewis细胞。通过RT-PCR检测mPer1在Lewis细胞中的表达;采用Chemico公司QCM(tm)24-Well Colorimetric Cell Migration Assay检测细胞迁移的变化。结果与pcDNA3.1空质粒相比,pcDNA3.1-mPer1转染组mPer1表达增加,其迁移率与对照组比较明显下降。结论节律基因mPer1过表达能够抑制Lewis肺癌细胞的迁移。Objective To study the effects of circadian gene mPeriod1 （roPer1） on migration of Lewis lung cancer cells by mPer1-overexpression. Methods Lipofectamine-mediated gene transfection method was used to transfect roper1 gene into Lewis lung cancer cells. The expression of roper1 gene was detected by RT-PCR and Western blotting. The cell migration was detected by Chemico QCM（tm） 24-Well Colorimetric Cell Migration Assay. Results As compared with the cells transfected with pcDNA3.1, the cells transfected with pcDNA3, 1-roPer1 demonstrated the low migration. Conclusion The overexpressed roper1 may inhibit the migration of Lewis lung carcinoma cells.

关 键 词：mPer1基因 细胞迁移 Lewis细胞 近日节律 

分 类 号：R73-362[医药卫生—肿瘤]