复合营养素对应激大鼠血清IL-6水平的影响  

Effects of compound nutrients on changes in serum stress-induced IL-6 levels in rats

作　　者：刘亚莉[1] 迟素敏[1] 毕辉[1] 樊荣[1] 王跃民[1] 陈耀明[2] 骆文静[2] 陈景元[2] 裴建明[1] 

机构地区：[1]第四军医大学生理学教研室,西安710032 [2]军事预防医学系劳动与环境卫生学教研室

出　　处：《解放军医学杂志》2007年第8期836-838,共3页Medical Journal of Chinese People's Liberation Army

基　　金：全军医药卫生科研基金重大项目(062047);第四军医大学军事医学课题(05XJM002)

摘　　要：目的探讨复合营养素对大鼠应激时血清细胞因子白介素6(IL-6)水平的影响。方法36只SD大鼠随机分为急性应激和慢性应激两组,其中各自又分为正常对照组、应激对照组、应激+复合营养紊组。急性应激组束缚冷水浸泡应激30min,应激后0、0.5、1和2h处死动物取血;慢性应激组束缚冷水浸泡每日应激1次,30min,持续2周,最后1次应激结束后30min处死大鼠取血。ELISA检测大鼠血清中的IL-6水平。结果束缚冷水浸泡应激可引起IL-6水平升高,而应用复合营养素对此有抑制作用。其中急性应激组应激后30min的变化最显著,IL-6水平由17.69±0.96pg/ml升高至29.67±1.64pg/ml,升高了67.8%;应用复合营养素可减轻应激所引起的IL-6水平的升高,30min时间点降至22.30±2.52pg/ml,降低了24.9%。慢性应激组其应激对照组的IL-6水平升高至30.69±1.08pg/ml,应激+复合营养素组则降低至22.70±1.68pg/ml。结论复合营养素可以显著改善应激大鼠的内分泌免疫调节功能,对减轻应激所造成的损伤可能具有一定作用。Objective To investigate the effects of compound nutrients on serum concentrations of IL-6 induced by immobilization and cold water-immersion in rats. Methods Stress was produced by immobilization and cold water-immersion. Rats were randomly assigned to two groups, i.e. acute and chronic stress groups. Acute stress group was divided again into normal control group （C）, acute stress group （AS） and acute stress ＋ compound nutrients group （AS＋CN）. Chronic stress group was assigned again into normal control group （C）, chronic stress group （CS） and chronic stress ＋ compound nutrients group （CS＋CN）. The serum IL-6 level was determined by enzyme-linked immunosorbent assay （ELISA）. Results Oral （gavage） administration of compound nutrients may attenuate effect of stress induced by acute and chronic immobilization, and reduce the increase in serum IL-6 level induced by cold water-immersion stress. In acute immobilization and cold water-immersed stress group, the serum IL-6 level rose significantly from 17. 69±0.96 to 29.67±1.64pg/ml 30 minutes after stress, and the administration of compound nutrients effectively attenuated the rise in serum IL-6 level 30 minutes after stress from 29. 67±1.64 to 22. 30±2. 52pg/ml （P〈0. 01）. The serum IL-6 level rose significantly to 30. 7±1.08 pg/ml 2 weeks after immobilization and cold water-immersion, and in the group of stress plus compound nutrients, the serum IL-6 level lowered significantly to 22. 7±1. 68pg/ml （P〈0. 01）. Conclusion The result suggest that compound nutrients might be proposed as a possible agent to modulate stress-related disorders.

关 键 词：复合营养素 白细胞介素6 应激 

分 类 号：R151[医药卫生—营养与食品卫生学]