天然牛磺酸对肝纤维化大鼠TGF-β_1/Smad信号通路的影响  

Effects of natural taurine on TGF-β_1/Smad singaling pathway in rats of hepatic fibrosis

作　　者：邓鑫[1] 梁健[1] 黄彬[1] 李益忠[1] 覃黎葵[1] 张锡流[2] 

机构地区：[1]广西中医学院附属瑞康医院肝胆内科,广西南宁530011 [2]广西中医学院第一附属医院病理科,广西南宁530023

出　　处：《大连医科大学学报》2007年第4期336-339,共4页Journal of Dalian Medical University

基　　金：广西自然科学基金(0144016)

摘　　要：[目的]探讨天然牛磺酸对肝纤维化大鼠TGF-β1/Smad信号通路的影响。[方法]Wistar大鼠随机分成对照组、模型组、天然牛磺酸治疗组,采用CCL4诱导肝纤维化模型。酶联免疫吸附法(ELISA)测定血清TGF-β1含量;免疫组织化学法检测肝组织TGF-β1、Smad3、Smad7蛋白表达;RT-PCR检测肝组织TGF-β1、TβRI和TβRIImRNA表达;分别用HE染色和Masson染色。肝组织观察病理组织变化。[结果]治疗组血清TGF-β1含量为(1.65±0.32)μg/L,显著低于模型组(3.93±0.32)μg/L,P<0.01;治疗组肝组织TGF-β1,Smad3蛋白表达分别为(4.5±0.6)%、(2.5±0.8)%,显著低于模型组(7.5±1.2)%、(3.9±0.8)%,P<0.05,Smad7为(6.2±1.3)%,显著高于模型组(1.3±0.2)%,P<0.01;治疗组肝组织TGF-β1,TβRI和TβRIImRNA表达分别为0.83±0.21、0.45±0.16、0.63±0.15,显著低于模型组1.62±0.45、0.93±0.24、1.25±0.45,P<0.01;治疗组肝纤维化分期积分为2.07±0.31,显著低于模型组3.18±0.42,P<0.01。[结论]天然牛磺酸能抑制TGF-β1及其受体和Smad3的表达,上调Smad7的表达,具有抑制肝纤维化的作用。[ Objective] To investigate the Effects of natural taurine on TGF - β1/Smad singaling pathway in rats of hepatic fibrosis. [ Methods ] Wistar rats were randomly divided into normal group, model group and natural taurine treatment group （each group, n = 20） ,hepatic fibrosis model were induced by CCL4. The serum concentration of TGF - β1 was assayed with ELISA. The protein expression of TGF- β1, Smad4, Smad7, with immunohis- stochemistry method. The gene expression of TGF- β1,TβRⅠ and TβRⅡ with PR - PCR method. Histopathological changes in rat＇ s liver tissue were detected with HE and Masson collagen staining. [ Results ] Treatment group of serum concentration of TGF - β1 were （ 1.65 ±0.32 ） μg/L , being lower than that in model group （3.93 ± 0.32 ） μg/L, P 〈 0.01, the protein expression of TGF - β1, Smad3 were （4.5 ± 0.6 ） %, （2.5 ± 0.8 ） % respectively, being lower than that in model group （7.5 ± 1.2）% , （3.9 ±0.8）% ,P 〈0.05, Smad7 were （6.2 ± 1.3）%, being higher than that in model group （1.3 ± 0.2） % ,P 〈 0.01, the gene expression of TGF - β1, TβRⅠ , TβRⅡ were 0.83 ± 0.21,0.45 ± 0.16, 0.63 ± 0.15 respectively,being lower than that in model group 1.62 ± 0.45,0.93 ±0.24, 1.25 ±0.45 ,P 〈0.01. Treatment group of hepatic fibrosis score were 2.07 ± 0.31, being lower than that in model group 3.18 ± 0.42, P 〈 0.01. [ Conclusion ] Natural taurine can inhibit the expression TGF- β1 and it＇s receptor,and increase the expression Smad7 and can inhibit hepatic fibrosis.

关 键 词：牛磺酸 肝纤维化 转化生长因子-Β1 SMAD 

分 类 号：R575.2[医药卫生—消化系统]