缺血再灌注对肝癌组织及正常肝组织的损伤  

The effect of ischemia and reperfusion injury to hepatocarcinoma and normal liver tissues

作　　者：张岚[1] 杨侠[2] 杨宪法[2] 赵佐庆[3] 

机构地区：[1]解放军第三医院理疗科,陕西宝鸡721004 [2]第四军医大学唐都医院教务科,陕西西安710038 [3]第四军医大学唐都医院实验外科,陕西西安710038

出　　处：《中国普通外科杂志》2007年第8期783-785,共3页China Journal of General Surgery

摘　　要：目的研究缺血再灌注(IR)对肝癌和正常肝组织的损伤作用。方法超声引导穿刺新西兰兔肝脏左中叶注射VX2肿瘤组织混悬液,建立肝脏肿瘤模型。2周后,证实荷瘤模型已成功构建,乃剖腹阻断肝左中叶的肝动脉分支,60 min后去除血管阻断恢复血流;分别于再灌注0 min,1 h,1 d,3 d和7 d取肝癌组织和正常肝组织石蜡包埋切片,以HE染色法和荧光脱氧核苷酸末端转移酶介导的dUTP缺口末端标记(TUNEL)法观察两种组织细胞凋亡情况。结果HE染色显示,IR后两种组织的凋亡细胞均有增加。至1 d时凋亡达峰值(肝癌为23%,正常组织为10%),其后虽有降低,但至7d时仍明显高于IR前水平(P<0.01)。以癌组织细胞凋亡最为显著,其在各时点阳性细胞率均明显高于正常肝组织。TUNEL实验显示,IR前癌组织凋亡细胞(5%)即多于正常肝脏组织(1%)。IR后,癌组织和正常肝组织凋亡改变的特点与HE染色相似,于IR1 d和7 d凋亡细胞率分别为25%,15%和8%,2%。结论IR后肝癌组织的损伤和细胞凋亡较正常肝组织更为显著。Objective To study the effect of ischemia and reperfusion injury to hepatocarcinoma and normal liver tissues. Methods The hepatocarcinoma animal models were established by the uhrasonography-guided implantation of VX2 tumor tissue suspension into the left-middle lobe of liver of rabbits. Two weeks later, the established hepotocarcinoma animal modal and the control group animal were subjected to 60 minutes clamp of the left-middle lobe artery branch followed by reperfusion at 0 min, 1 h, 1 d, 3 d and 7 d respectively. Apoptotic changes in the hepatocarcinoma and normal hepatic tissues were observed by means of HE staining and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling （TUNEL）. Results The result of HE staining indicated that the number of apoptotic cells in hepatocarcinoma and normal liver tissues increased to the highest point 1 d following reperfusion （23%, 10% ）, but even though the positive cell count decreased to a certain extent until 7 d after reperfusion, it was still more obvious than that before the reperfusion. The positive apoptosis ratio of hepatocarcinoma tissue retained a higher level than that of normal liver tissues at all time points..The result of TUNEL showed that the number of the apoptotic cells in hepatocarcinoma tissues （ 5 % ） was larger than that of normal liver tissues before ischemia （ 1% ）. The apoptotic characteristics of hepatocarcinoma and normal liver tissues after reperfusion were similar to those of HE staining and their apoptotic ratio was 25%, 15% and 8%, 2% after reperfusion for 1 d and 7 d respectively. Conclusions Ischemia and reperfusion can cause more intense injury and apoptosis in hepatocarcinoma tissue than in normal hepatic tissues.

关 键 词：肝肿瘤 缺血再灌注损伤 细胞凋亡 疾病模型 动物 兔 

分 类 号：R735.7[医药卫生—肿瘤]