大孔载体固定化脂肪酶  

Investigation of Immobilized Lipase onto Macroporous Carrier

作　　者：蔡宏举[1] 付大雁[1] 王满意[1] 周鑫[1] 谭天伟[1] 

机构地区：[1]北京化工大学生命科学与技术学院,北京市生物加工过程重点实验室,北京100029

出　　处：《过程工程学报》2007年第4期773-777,共5页The Chinese Journal of Process Engineering

基　　金：国家自然科学基金资助项目(编号:20636010;50373003);国家杰出青年基金资助项目(编号:20325622);北京市科技计划基金资助项目(编号:D0205004040211)

摘　　要：用自制大孔载体固定化脂肪酶,对固定化条件进行了优化,比较了固定化酶与游离酶的酶学参数.结果表明,酶粉与载体质量比为1:1、固定化温度在20～25℃之间、固定化时间1.5h的条件下,所得固定化酶的酶活最高.固定化酶的最适pH为8.5,最适温度为40℃,其热稳定性、操作稳定性都比游离酶高,4℃下保存7d后,酶活仍剩余94%.Immobilization of lipase on macroporous poly（glycidyl methacrylate-co-divinylbenzene） was investigated and followed by the optimization of immobilizing conditions. The optimized results were achieved with the 1：1 mass ratio of lipase to the carrier, coupling temperature between 20-25 ℃, and immobilizing time of 1.5 h. The optimum pH and temperature of immobilized lipase were 8.5 and 40 ℃, respectively. The activity of immobilized lipase remained 94% of the origin after storage at 4 ℃ for 7 d. The experimental results showed that the thermal stability and operational stability were improved in comparison with free lipase.

关 键 词：大孔载体 脂肪酶 固定化酶 

分 类 号：Q814.2[生物学—生物工程]