牛磺酸对苯并(a)芘致人胚肝细胞损伤拮抗作用  

Protective effect of taurine against DNA damage of human derived fetal hepatocytes induced by Benzo(a) Pyrene

作　　者：鲁力[1] 秦秋兰[2] 肖德强[1] 王志清[1] 邹亚玲[3] 鲁文清[3] 

机构地区：[1]广西医科大学公共卫生学院营养科学研究中心,南宁530021 [2]广西疾病预防控制中心 [3]华中科技大学同济医学院公共卫生学院

出　　处：《中国公共卫生》2007年第9期1131-1133,共3页Chinese Journal of Public Health

基　　金：广西自然科学基金(桂科自0640115)

摘　　要：目的探讨牛磺酸对苯并(a)芘致肝细胞DNA损伤的作用和机制。方法以人胚肝细胞为靶细胞体外培养,按完全随机实验设计分成5个组:(1)空白对照组;(2)溶剂对照组;(3)苯并(a)芘组:25μmol/L苯并(a)芘加入细胞培养体系后培养2 h;(4)牛磺酸组:设0.5,1.0,2.0,4.0μmol/L 4个浓度,加入培养体系后培养24 h;(5)牛磺酸预防组:先以4个不同浓度的牛磺酸预处理24 h,再加入25μmol/L的苯并(a)芘,培养2 h。各组培养结束后用单细胞凝胶电泳技术(SCGE)检测细胞DNA损伤,用分光光度法测定细胞培养液中丙二醛(MDA)、还原型谷胱甘肽(GSH)、天门冬酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)含量。结果(1)微量苯并(a)芘可致人胚肝细胞NDA明显损伤,同时使MDA、ALT、AST水平升高、GSH含量降低,牛磺酸的作用则相反;(2)牛磺酸预处理可明显减轻苯并(a)芘引起的人胚肝细胞DNA损伤,抑制MDA、AST、ALT含量升高和GSH含量下降,其作用呈剂量-效应关系;(3)人胚肝细胞DNA损伤的Oliver尾矩值与细胞培养液中MDA、AST、ALT含量呈明显正相关,与GSH含量呈明显负相关。结论苯并(a)芘致人胚肝细胞DNA损伤可能与氧化损伤有关,而牛磺酸具有拮抗苯并(a)芘致人胚肝细胞DNA损伤的良好作用。Objective To investigate the protective effect of taurine on DNA damages of human derived fetal hepatocytes induced by B（a）P. Methods Human derived fetal hepatocytes （L-02 cells）were randomized into 5 groups： （ Ⅰ ） blank control group; （ Ⅱ ） solvent control group; （ Ⅲ ） B（a） P group explored to B（a） P at the concentration of 25 μmol/L, in culture medium for 2 hours： （Ⅳ） Taurine group explored to taurine at the concentration of 0.5, 1.0, 2.0, 4.0 mmol/L in culture medium for 24 hours： （Ⅴ） Taurine preventive group explored to taurine at 4 different concentration in culture medium for 24 hours and treated further for 2 hours by adding 25 μmol/L B（a）P into the culture media. Single cell gel etectrophoresis （SCGE） were used to detect DNA damage. The concentration of MDA, GSH, AST, ALT in the supernatants of culture media were tested by spectrophotometric method to reflect the oxidative damage level of cell. Results （ 1）Only a low concentration of B（ a）P could induce DNA damage of L- 02 cells and cause the concentration of MDA, AST, ALT in the supernatant increased and GSH decreased. （2）Pretreatment with taurine could antagonize significantly the toxicity of L- 02 cells induced by B（a）P. It could reduce the MDA, AST, ALT level and increase GSH content in cells in doseeffect manner. （3） The DNA damage was positive correlation with MDA, AST and ALT levels. However, it was negative correlation with GSH level. Conclusion Oxidative damage might be a important mechanism with regard to DNA damage of human derived feral heparocytes induced by B（a）P; Taurine could play a protective role in hepatic cell damage by means of antoxidation effect.

关 键 词：牛磺酸 苯并(A)芘 人胚肝细胞 DNA损伤 单细胞凝胶电泳(SCGE) 

分 类 号：R15[医药卫生—营养与食品卫生学]