聚-ε-赖氨酸补料发酵的初步研究  

Studies on fed-batch fermentation of poly-ε-L-lysine

作　　者：陈雄[1] 章莹[1] 袁金凤[1] 王实玉[1] 王金华[1] 

机构地区：[1]湖北工业大学生物工程学院湖北省工业微生物重点实验室,武汉430068

出　　处：《工业微生物》2007年第4期20-22,共3页Industrial Microbiology

基　　金：湖北省教育厅青年项目(Q200514001)

摘　　要：在5L自动发酵罐中,通过分批发酵和补料分批发酵,对白色链霉菌(Strepomyces albulus)生物合成聚-ε-赖氨酸(ε-PL)进行了初步研究。结果表明,在分批发酵中,利用pH控制策略,ε-PL产量从0.6 g/L提高到2.4 g/L。在分批补料发酵中,采用pH控制策略,当糖浓度在10g/L以下,补加葡萄糖和硫酸铵,则菌体大量生长,ε-PL产量提高到16.81 g/L,为分批培养的27倍。The fermentation of poly-ε-L-lysine （ε-PL） from Streptomyces albulus was investigated by batch culture and fed-batch culture in 5 liter fermentor. The results showed that the ε-PL yield enhanced from 0.6 g/L to 2.4 g/L with pH- control strategy at batch culture. In the course of fed-batch culture, when glucose concentration in broth was below 10 g/ L, glucose and （NH4）2SO4 were fed into the broth. With pH-control and fed-batch strategy, ε-PL yield reached 16.81 g/L, 27 times higher than that of batch culture without pH control.

关 键 词：白色链霉菌 分批发酵 补料发酵 聚-ε-赖氨酸 

分 类 号：TQ922.3[轻工技术与工程—发酵工程]