对产油脂酵母的细胞破碎方法及油脂提取效果的比较  

COMPARISON OF CELL DISRUPTION METHODS AND LIPID EXTRACTION RATE OF LIPID-PRODUCING YEAST

作　　者：孙晓璐[1] 孙玉梅[1] 曹芳[1] 华艳艳[2] 赵宗保[2] 

机构地区：[1]大连工业大学生物与食品工程学院,辽宁大连116034 [2]中国科学院大连物理化学研究所,辽宁大连116023

出　　处：《河南工业大学学报（自然科学版）》2007年第4期67-69,共3页Journal of Henan University of Technology：Natural Science Edition

基　　金：国家973项目(2004CB719703)

摘　　要：研究了冻融破碎法、超声波破碎法、酸热法以及冻融和酸热结合法破碎发酵性丝孢酵母(Trichosporon fermentans)细胞,并考察了乙醚-石油醚法和氯仿-甲醇法萃取细胞内油脂.实验结果表明,采用酸热法破碎细胞后以乙醚-石油醚作为萃取溶剂油脂提取效果较好,油脂得率高达29.8%.Trichosporon fermentans were disrupted by freeze-meh, uhrasonic wave, acid-heating method and freeze-meh ＆ acid-heating method respectively. The lipid extraction rates of Trichosporon ferments by the ether-benzine and chloroform-methanol were compared. The results implied that the ceils were disrupted by acid-heating and then extracted by the ether-benzine, by this procedure, the lipid extraction rate could reach higher level as high as 29.8%.

关 键 词：发酵性丝孢酵母 细胞破碎 油脂 提取 

分 类 号：TS201.3[轻工技术与工程—食品科学]