COX-2在肝细胞肝癌中的表达及其与细胞凋亡的关系  

RELATIONSHIP BETWEEN THE EXPRESSION OF COX-2 AND CELL APOPTOSIS IN HEPATOCELLUAR CARCINOMA

作　　者：虞长春[1] 吴正升[2] 吴强[2] 丁向东[2] 杨枫[2] 

机构地区：[1]安徽省望江县人民医院普外科,望江246203 [2]安徽医科大学病理学教研室

出　　处：《肝胆外科杂志》2007年第4期305-307,共3页Journal of Hepatobiliary Surgery

摘　　要：目的探讨肝细胞癌(HCC)中环氧化酶-2(COX-2)的表达与细胞凋亡的关系及其可能的机制。方法选用44例HCC组织制作组织芯片,应用免疫组织化学S-P法检测癌组织COX-2、caspase-3和survivin的表达情况。应用末端标记法(TUNEL)检测细胞凋亡指数(AI)。结果HCC中COX-2蛋白阳性率为52.3%,其表达与AI呈显著负相关(r=-0.354,P<0.05),与caspase-3蛋白表达也呈显著负相关(r=-0.447,P<0.01),但与survivin蛋白的表达呈显著正相关(r=0.394,P<0.01)。AI与caspase-3呈显著正相关(r=0.381,P<0.05),但与survivin呈显著负相关(r=-0.458,P<0.01)。结论本研究证实了HCC中COX-2的抗肿瘤细胞凋亡作用;其机制可能是通过上调肿瘤细胞survivin表达,同时下调caspase-3表达途径实现的。Objective To study the relationship between the expression of cyclooxygenase-2 （COX-2） and apoptosis in hepatocelluar carcinoma （HCC） , and to investigate its possible mechanisms. Methods The protein expression of COX-2, caspase-3 and survivin was detected by immunohistochemical method, and cell apoptosis was examinated by TUNEL technique in tissue microarrys constructed with 44 cases of HCC. Results COX-2 expressed in 52. 3% of 44 HCC cases, which was negatively correlated with apoptotic indices （AI） （ r = - 0. 354, P 〈 0. 05 ） and caspase-3 expression （ r = - 0. 447, P 〈 0. 01 ）, but positively correlated with survivin （ r = 0. 394, P 〈 0.01 ）. AI was positively correlated with the expression of caspase-3 （ r = 0. 381, P 〈 0. 05 ） , but negatively with that of survivin（ r = -0. 458 ,P 〈 0. 01 ）. Conclusion COX-2 might play a significant role in the anti-apoptosis effect of HCC cells via up-regulating survivin expression and down-regulating caspase-3 expression.

关 键 词：肝细胞癌 COX-2 CASPASE SURVIVIN 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]